Mwakigonja Amos R, Pak Fatemeh, Pyakurel Pawan, Mosha Innocent J, Urassa Willy K, Kaaya Ephata E, Biberfeld Peter
Immunopathology Laboratory, Department of Oncology-Pathology, Cancer Centrum Karolinska, R8:00, Karolinska Hospital/Institute, S-171 76 Stockholm, Sweden.
Oncol Rep. 2007 Jun;17(6):1291-9.
Oral Kaposi's sarcoma (OKS) from Tanzanian patients (78) at Muhimbili National Hospital/Muhimbili University College of Health Sciences corresponding to approximately 10% of KS registered during 1990-2005, were diagnosed (ELISA) as HIV-infected (OAKS) (74/78) and endemic KS (4/78). Females were 69.2% (54/78) with median age 31 and males 30.8% (24/78) with median age 38. More males (50%) had systemic KS than females (37%) and 4-times more multicentric OKS. All tested (34) oral KS patients sera had HHV-8 antibodies. Available (31/78) blood showed very low CD4+ T-lymphocyte counts. Most OKS (61.5%) had nodular histology. Immunostaining showed adult male nodular OAKS to have a significantly higher frequency of viral LANA+, endothelial CD34+ tumour spindle-cells (SC) and more Ki-67+ (median =24.1%) proliferating cells compared to females (17.2%). Juvenile nodular OAKS had more LANA+ and Ki-67+ cells than corresponding adult cases. Significantly more LANA+ and Ki-67+ cells were found in nodular OAKS compared to cutaneous HIV/AIDS Kaposi's sarcoma (CAKS). A positive correlation (60%) was found between the proliferation index (Ki-67+ cell frequency) and LANA+/CD34+ SC. OKS in Tanzania is since 1990, mostly seen in females, associated with HIV/AIDS and advanced (nodular) histopathology. Males have more systemic tumour burden while more females develop primary OAKS. HHV-8+ cells were more frequent in nodular male than female and in juvenile than adult nodular OAKS than cAKS. Higher tumoral HHV-8 content appeared to be correlated to proliferation index.
在穆希姆比利国家医院/穆希姆比利大学健康科学学院，对来自坦桑尼亚的78例口腔卡波西肉瘤（OKS）患者进行了研究，这些病例约占1990年至2005年期间登记的卡波西肉瘤病例的10%。通过酶联免疫吸附测定（ELISA）诊断，其中74例为HIV感染相关的口腔卡波西肉瘤（OAKS），4例为地方性卡波西肉瘤。女性占69.2%（54/78），中位年龄31岁；男性占30.8%（24/78），中位年龄38岁。患有系统性卡波西肉瘤的男性（50%）比女性（37%）更多，多中心性OKS患者中男性是女性的4倍。所有接受检测的34例口腔卡波西肉瘤患者血清均含有HHV - 8抗体。可获取的31份血液样本显示CD4 + T淋巴细胞计数极低。大多数OKS（61.5%）具有结节状组织学特征。免疫染色显示，成年男性结节状OAKS中病毒LANA +、内皮细胞CD34 +肿瘤梭形细胞（SC）以及增殖的Ki - 67 +细胞（中位数=24.1%）的频率显著高于女性（17.2%）。青少年结节状OAKS中的LANA +和Ki - 67 +细胞比相应的成年病例更多。与皮肤HIV/AIDS相关的卡波西肉瘤（CAKS）相比，结节状OAKS中发现的LANA +和Ki - 67 +细胞明显更多。增殖指数（Ki - 67 +细胞频率）与LANA +/CD34 + SC之间存在正相关（60%）。自1990年以来，坦桑尼亚的OKS大多见于女性，与HIV/AIDS相关且具有晚期（结节状）组织病理学特征。男性的系统性肿瘤负担更重，而更多女性发生原发性OAKS。结节状男性OAKS中的HHV - 8 +细胞比女性更多，青少年结节状OAKS中的HHV - 8 +细胞比成年患者更多，且比CAKS更多。肿瘤中更高的HHV - 8含量似乎与增殖指数相关。
