盐类对耐盐酵母尼泊尔德巴利酵母NCYC 3413生长及果胶酶产生的影响
Effect of salts on growth and pectinase production by halotolerant yeast, Debaryomyces nepalensis NCYC 3413.
作者信息
Gummadi Sathyanarayana N, Kumar Sawan, Aneesh C N A
机构信息
Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600 036, India.
出版信息
Curr Microbiol. 2007 Jun;54(6):472-6. doi: 10.1007/s00284-007-0060-y. Epub 2007 May 8.
In this study, Debaryomyces nepalensis NCYC 3413 isolated from rotten apple was studied for its halotolerance and its growth was compared with that of Saccharomyces cerevisiae in high salt medium. The specific growth rate of D. nepalensis was not affected by KCl even up to a concentration of 1 M: , whereas NaCl and LiCl affected the growth of D. nepalensis. Among all tested salts, LiCl showed maximum inhibition on growth. At all conditions, halotolerance of D. nepalensis was much higher than that of S. cerevisiae. D. nepalensis showed maximum viability (80-100%) when grown in KCl, which was higher than with NaCl and LiCl. Pectinase production by D. nepalensis was noted at all high salt concentrations, namely, 2 M NaCl, 2 M KCl, and 0.5 M LiCl, and the maximum specific activity was observed when the strain was grown in 2 M NaCl.
在本研究中，对从腐烂苹果中分离出的尼泊尔德巴利酵母NCYC 3413的耐盐性进行了研究，并将其在高盐培养基中的生长情况与酿酒酵母进行了比较。尼泊尔德巴利酵母的比生长速率即使在KCl浓度高达1 M时也不受影响，而NaCl和LiCl会影响尼泊尔德巴利酵母的生长。在所有测试的盐中，LiCl对生长的抑制作用最大。在所有条件下，尼泊尔德巴利酵母的耐盐性远高于酿酒酵母。当在KCl中生长时，尼泊尔德巴利酵母显示出最高的活力（80 - 100%），高于在NaCl和LiCl中的情况。在所有高盐浓度下，即2 M NaCl、2 M KCl和0.5 M LiCl中，均观察到尼泊尔德巴利酵母产生果胶酶，当该菌株在2 M NaCl中生长时，观察到最大比活性。
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