Soriano Margarita, Diaz Pilar, Pastor F I Javier
Department of Microbiology, Faculty of Biology, University of Barcelona, Avenida Diagonal 645, 08028 Barcelona, Spain.
Curr Microbiol. 2005 Feb;50(2):114-8. doi: 10.1007/s00284-004-4382-8. Epub 2005 Feb 8.
Strains Paenibacillus sp. BP-23 and Bacillus sp. BP-7, previously isolated from soil from a rice field, secreted high levels of pectinase activity in media supplemented with pectin. Production of pectinases in strain Paenibacillus sp. BP-23 showed catabolite repression, while in Bacillus sp. BP-7 production of pectin degrading enzymes was not negatively affected by glucose. The two strains showed lyase activities as the predominant pectinases, while hydrolase activity was very low. Analysis of Paenibacillus sp. BP-23 in SDS-polyacrylamide gels and zymograms showed five pectinase activity bands. The strict requirement of Ca(2+) for lyase activity of the strain indicates that correspond to pectate lyases. For Bacillus sp. BP-7, zymograms showed four bands of different size. The strain showed a Ca(2+) requirement for lyase activity on pectate but not on pectin, indicating that the pectinolytic system of Bacillus sp. BP-7 is comprised of pectate lyases and pectin lyases. The results show differences in pectin degrading systems between the two aerobic sporogenous bacterial strains studied.
菌株芽孢杆菌属BP - 23和芽孢杆菌属BP - 7先前从稻田土壤中分离得到，在添加果胶的培养基中能分泌高水平的果胶酶活性。芽孢杆菌属BP - 23菌株中果胶酶的产生表现出分解代谢物阻遏，而在芽孢杆菌属BP - 7中，果胶降解酶的产生不受葡萄糖的负面影响。这两种菌株以裂解酶活性作为主要的果胶酶，而水解酶活性非常低。在十二烷基硫酸钠 - 聚丙烯酰胺凝胶和酶谱中对芽孢杆菌属BP - 23的分析显示有五条果胶酶活性带。该菌株裂解酶活性对Ca(2+)的严格需求表明其对应于果胶酸裂解酶。对于芽孢杆菌属BP - 7，酶谱显示有四条不同大小的带。该菌株对果胶酸裂解酶活性有Ca(2+)需求，但对果胶没有，这表明芽孢杆菌属BP - 7的果胶分解系统由果胶酸裂解酶和果胶裂解酶组成。结果表明在所研究的两种需氧产芽孢细菌菌株之间果胶降解系统存在差异。
