Fu Lung-Ming, Leong Jik-Chang, Lin Chiu-Feng, Tai Chang-Hsien, Tsai Chien-Hsiung
Department of Materials Engineering, National Pingtung University of Science and Technology, Pingtung, Taiwan 912.
Biomed Microdevices. 2007 Jun;9(3):405-12. doi: 10.1007/s10544-007-9049-3.
This paper presents a novel microfluidic capillary electrophoresis (CE) device featuring a double-T-form injection system and an expansion chamber located at the inlet of the separation channel. This study addresses the principal material transport mechanisms depending on parameters such as the expansion ratio, the expansion length, the fluid flow. Its design utilizes a double-L injection technique and combines the expansion chamber to minimize the sample leakage effect and to deliver a high-quality sample plug into the separation channel so that the detection performance of the device is enhanced. Experimental and numerical testing of the proposed microfluidic device that integrates an expansion chamber located at the inlet of the separation channel confirms its ability to increase the separation efficiency by improving the sample plug shape and orientation. The novel microfluidic capillary electrophoresis device presented in this paper has demonstrated a sound potential for future use in high-quality, high-throughput chemical analysis applications and throughout the micro-total-analysis systems field.
本文介绍了一种新型微流控毛细管电泳（CE）装置，其具有双T形进样系统和位于分离通道入口处的扩展腔。本研究探讨了取决于诸如扩展比、扩展长度、流体流动等参数的主要物质传输机制。其设计采用双L进样技术并结合扩展腔，以最小化样品泄漏效应，并将高质量的样品塞输送到分离通道中，从而提高装置的检测性能。对所提出的在分离通道入口处集成扩展腔的微流控装置进行的实验和数值测试证实了其通过改善样品塞的形状和取向来提高分离效率的能力。本文介绍的新型微流控毛细管电泳装置在高质量、高通量化学分析应用以及整个微全分析系统领域展现出良好的未来应用潜力。
