Fu Lung-Ming, Lin Che-Hsin
Graduate Institute of Materials Engineering, National Pingtung University of Science and Technology, Pingtung, Taiwan, 912.
Anal Chem. 2003 Nov 1;75(21):5790-6. doi: 10.1021/ac034667q.
This paper presents an experimental and numerical investigation into the use of low-leakage injection techniques to deliver sample plugs within electrophoresis microchips. The study addresses the principal material transport mechanisms such as electrokinetic migration, fluid flow, and diffusion and gives detail analyses to the double-L injection technique, which employs electrokinetic manipulations to avoid sample leakage within the microchip. Electrical potential contour, velocity vector, and streamline distribution in the micro CE chip are successfully developed. Experimental and numerical testing results show the double-L injection technique is capable of reducing sample leakage within cross-form microfluidic chips. The current study confirms the double-L injection technique has an exciting potential for use in high-quality, high-throughput chemical analysis applications and in many other applications throughout the field of micro total analysis systems.
本文介绍了一项关于在电泳微芯片中使用低泄漏进样技术输送样品塞的实验和数值研究。该研究探讨了诸如电动迁移、流体流动和扩散等主要物质传输机制，并对双L进样技术进行了详细分析，该技术采用电动操作以避免样品在微芯片内泄漏。成功绘制了微CE芯片中的电势等高线、速度矢量和流线分布。实验和数值测试结果表明，双L进样技术能够减少十字形微流控芯片内的样品泄漏。当前研究证实，双L进样技术在高质量、高通量化学分析应用以及整个微全分析系统领域的许多其他应用中具有令人兴奋的潜力。
