Fu Lung-Ming, Yang Ruey-Jen, Lee Gwo-Bin, Pan Yu-Jen
Graduate Institute of Materials Engineering, National Pingtung University of Science and Technology, Pingtung, Taiwan.
Electrophoresis. 2003 Sep;24(17):3026-32. doi: 10.1002/elps.200305528.
This paper presents an experimental and numerical investigation into electrokinetic focusing flow injection for bioanalytical applications on 1 x N (i.e., 1 sample inlet port and N outlet ports) and M x N (i.e., M sample inlet ports and N outlet ports) microfluidic chips. A novel device is presented which integrates two important microfluidic phenomena, namely electrokinetic focusing and valveless flow switching within multiported microchannels. The study proposes a voltage control model which achieves electrokinetic focusing in a prefocusing sample injection system and which allows the volume of the sample to be controlled. Using the developed methods, the study shows how the sample may be prefocused electrokinetically into a narrow stream prior to being injected continuously into specified outlet ports. The microfluidic chips presented within this paper possess an exciting potential for use in a variety of techniques, including high-throughput chemical analysis, cell fusion, fraction collection, fast sample mixing, and many other applications within the micrototalanalysis systems field.
本文介绍了一种针对1×N（即1个样品入口和N个出口）以及M×N（即M个样品入口和N个出口）微流控芯片上生物分析应用的电动聚焦流动注射的实验和数值研究。文中展示了一种新型装置，该装置在多端口微通道内集成了两种重要的微流控现象，即电动聚焦和无阀流动切换。该研究提出了一种电压控制模型，该模型可在预聚焦样品进样系统中实现电动聚焦，并能控制样品体积。利用所开发的方法，该研究展示了如何在将样品连续注入指定出口之前，通过电动方式将其预聚焦成窄流。本文所介绍的微流控芯片在多种技术中具有令人兴奋的应用潜力，包括高通量化学分析、细胞融合、馏分收集、快速样品混合以及微全分析系统领域内的许多其他应用。
