Wang S, Wang X H
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China.
Food Addit Contam. 2007 Jun;24(6):573-82. doi: 10.1080/02652030601134533.
Analytical methods for zeranol residues are reviewed. Zeranol was a widely used as an anabolic promoter, and it could give rise to very low residues in the edible tissues of food animals. Zeranol was officially banned in Europe due to safety concerns because of its potential carcinogenic and endocrine-disrupting biological activity. A few analytical methods for determination of zeranol are reported in the literature and most of the methods such as thin-layer chromatography (TLC), gas chromatography-mass spectrometry (GC/MS), high-performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC/MS) and immunoassay are reviewed in this paper. Specific aspects of analysing zeranol such as sample selection, sample handling, method selection and chromatographic conditions are discussed. The instrumental methods such as LC/MS and GC/MS provide sensitive and specific techniques, but are very laborious and expensive. These methods are suitable for confirmation but not for screening of large numbers of samples. A rapid, sensitive and specific assay is needed to detect positive samples in routine analysis, and immunoassay offers practical advantages over the conventional instrumental methods in rapid analysis of zeranol residues. Immunochemical methods such as enzyme-linked immunoabsorbant assay (ELISA) are simple, rapid and cost-effective, with adequate sensitivity and specificity to detect small molecules. This review can be considered as a basis for further research aimed at identifying the most efficient approaches for the analysis of zeranol.
对玉米赤霉醇残留的分析方法进行了综述。玉米赤霉醇曾被广泛用作合成代谢促进剂，它会在食用动物的可食用组织中产生极低的残留量。由于其潜在的致癌和内分泌干扰生物活性，出于安全考虑，玉米赤霉醇在欧洲已被正式禁止使用。文献中报道了一些测定玉米赤霉醇的分析方法，本文对其中的大多数方法，如薄层色谱法（TLC）、气相色谱 - 质谱联用法（GC/MS）、高效液相色谱法（HPLC）、液相色谱 - 质谱联用法（LC/MS）和免疫分析法进行了综述。讨论了分析玉米赤霉醇的具体方面，如样品选择、样品处理、方法选择和色谱条件。诸如LC/MS和GC/MS等仪器方法提供了灵敏且特异的技术，但非常繁琐且昂贵。这些方法适用于确证，但不适用于大量样品的筛查。在常规分析中需要一种快速、灵敏且特异的检测方法来检测阳性样品，而免疫分析法在玉米赤霉醇残留的快速分析中比传统仪器方法具有实际优势。免疫化学方法，如酶联免疫吸附测定法（ELISA），简单、快速且具有成本效益，具有足够的灵敏度和特异性来检测小分子。这篇综述可被视为进一步研究的基础，旨在确定分析玉米赤霉醇的最有效方法。
