Saravanakumar Duraisamy, Muthumeena Kannappan, Lavanya Nallathambi, Suresh Seetharaman, Rajendran Lingan, Raguchander Thiruvengadam, Samiyappan Ramasamy
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 641 003, Tamil Nadu, India.
Pest Manag Sci. 2007 Jul;63(7):714-21. doi: 10.1002/ps.1381.
Fluorescent pseudomonad strains Pf1, TDK1 and PY15 individually and in combination were evaluated against leaffolder, Cnaphalocrocis medinalis Guen., in rice under in vitro, glasshouse and field conditions. Among the various treatments used, a combination of Pf1, TDK1 and PY15 strains effectively reduced the incidence of leaffolder pest in rice plants to an extent comparable with chlorpyrifos-methyl. In addition, morphogenesis of the insect pest in all stages, larval, pupal and adult, was greatly affected by a combination of Pseudomonas Pf1, TDK1 and PY15 strains. Further, the induction of defence-related molecules was demonstrated. An increased accumulation of defence molecules such as chitinase and proteinase inhibitors was observed with a combined Pf1, TDK1 and PY15 treatment compared with all other treatments. Western blot analysis of chitinase revealed the extra induction of 18, 28 and 35 kDa isoforms in rice plants treated with a mixture of Pf1, TDK1 and PY15 strains against leaffolder pest. The study revealed that a combination of fluorescent pseudomonad strains affects the development of leaffolder pest by inducing defence molecules in rice plants which in turn enhance resistance to leaffolder attack.
在体外、温室和田间条件下，对荧光假单胞菌菌株Pf1、TDK1和PY15单独及组合使用进行了评估，以对抗水稻纵卷叶螟（Cnaphalocrocis medinalis Guen.）。在所用的各种处理中，Pf1、TDK1和PY15菌株的组合有效地将水稻植株上纵卷叶螟害虫的发生率降低到与甲基毒死蜱相当的程度。此外，假单胞菌Pf1、TDK1和PY15菌株的组合对害虫在幼虫、蛹和成虫所有阶段的形态发生都有很大影响。此外，还证明了防御相关分子的诱导。与所有其他处理相比，Pf1、TDK1和PY15联合处理观察到防御分子如几丁质酶和蛋白酶抑制剂的积累增加。几丁质酶的蛋白质印迹分析显示，在用Pf1、TDK1和PY15菌株混合物处理的水稻植株中，针对纵卷叶螟害虫额外诱导出了18 kDa、28 kDa和35 kDa的同工型。该研究表明，荧光假单胞菌菌株的组合通过诱导水稻植株中的防御分子来影响纵卷叶螟害虫的发育，进而增强对纵卷叶螟攻击 的抗性。
