通过肽核酸功能化金纳米管的杂交调制离子通量。一种定量无标记DNA分析的新方法。
Hybridization-modulated ion fluxes through peptide-nucleic-acid- functionalized gold nanotubes. A new approach to quantitative label-free DNA analysis.
作者信息
Jágerszki Gyula, Gyurcsányi Róbert E, Höfler Lajos, Pretsch Ernö
机构信息
Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szt. Gellért tér 4, H-1111 Budapest, Hungary.
出版信息
Nano Lett. 2007 Jun;7(6):1609-12. doi: 10.1021/nl0705438. Epub 2007 May 8.
Abstract
The inner walls of gold nanotubes, prepared by template synthesis in the nanopores of polycarbonate track etch membranes, have been chemically modified with peptide nucleic acid (PNA) and used for label-free quantification of complementary DNA sequences. Selective binding of DNA to the PNA-modified nanotubes is shown to decrease the flux of optically detected anionic markers through the nanotubes in a concentration-dependent manner. The strong dependence of the biorecognition-modulated ion transport through the nanopores on the ionic strength suggests a dominantly electrostatic exclusion mechanism of the ion flux decrease as a result of DNA binding to the PNA-modified nanopores.
摘要
通过在聚碳酸酯径迹蚀刻膜的纳米孔中进行模板合成制备的金纳米管内壁，已用肽核酸（PNA）进行了化学修饰，并用于对互补DNA序列进行无标记定量。DNA与PNA修饰的纳米管的选择性结合显示出以浓度依赖的方式降低了光学检测到的阴离子标记物通过纳米管的通量。生物识别调节的离子通过纳米孔的传输对离子强度的强烈依赖性表明，由于DNA与PNA修饰的纳米孔结合，离子通量降低的主要机制是静电排斥。
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