Wortmann Glenn, Hochberg Lisa P, Arana Byron A, Rizzo Nidia R, Arana Flora, Ryan Jeffrey R
Infectious Diseases Division, Walter Reed Army Medical Center, Washington, DC 20307-5001, USA.
Am J Trop Med Hyg. 2007 May;76(5):906-8.
The polymerase chain reaction (PCR) assay has been reported for the diagnosis of cutaneous leishmaniasis (CL). Real-time (RT) PCR offers several advantages over traditional PCR, including faster processing time and decreased risk of contamination. Enhanced portability is another benefit that expands the applicability of the assay. A portable RT-PCR assay was transported from the United States to Guatemala for comparison with traditional diagnostic modalities. With the clinical diagnosis of CL as the gold standard, RT-PCR was positive in 86% (37 of 43) versus 53% (20 of 38) for microscopy and 72% (28 of 39) for culture. Negative RT-PCR samples (6) were also negative by traditional diagnostic methods (although subsequently determined to be positive by a nested kDNA PCR). Sixty-four percent (9 of 14) of cases tested and negative by microscopy and/or culture were positive by RT-PCR. This study demonstrates that a RT-PCR assay can be successfully deployed to offer enhanced sensitivity for the diagnosis of CL.
聚合酶链反应（PCR）检测已被报道用于皮肤利什曼病（CL）的诊断。实时（RT）PCR与传统PCR相比具有多个优点，包括处理时间更快和污染风险降低。增强的便携性是该检测方法适用性得以扩展的另一优势。一种便携式RT-PCR检测方法从美国运到危地马拉，用于与传统诊断方法进行比较。以CL的临床诊断作为金标准，RT-PCR的阳性率为86%（43例中的37例），而显微镜检查为53%（38例中的20例），培养为72%（39例中的28例）。RT-PCR阴性样本（6份）通过传统诊断方法检测也为阴性（尽管随后通过巢式kDNA PCR确定为阳性）。经显微镜检查和/或培养检测为阴性的病例中，64%（14例中的9例）通过RT-PCR检测为阳性。这项研究表明，RT-PCR检测方法可以成功应用，以提高CL诊断的敏感性。
