Vahjen W, Taras D, Simon O
Institute of Animal Nutrition, Free University of Berlin, Bruemmerstr. 34, 14195 Berlin, Germany.
Curr Issues Intest Microbiol. 2007 Mar;8(1):1-7.
Sows and their piglets were fed a diet supplemented with or without the probiotic E. faecium NCIMB10415 (also known as SF68). Piglets were sacrificed 14, 28, 35 and 56 days after birth and DNA from intestinal segments was extracted and purified. A real time PCR assay was used to distinguish Enterococcus spp. (16s rDNA based), E. faecium (Efaafm gene), E. faecalis (Efaafs gene) as well as the probiotic strain (unique plasmid sequence). Extracts of autoclaved sow feces inoculated with E. faecium and E. faecalis cultures were used to calibrate real time PCR results. The probiotic strain was detected in 14 day old suckling piglets before the piglets had access to the starter diet. In piglets of the probiotic group, probiotic E. faecium cell counts were always a significant proportion of total E. faecium cells in stomach digesta (4-20%), however only a small fraction of the total Enterococcus spp. cell number on day 14 and 28 in all intestinal segments (0.1-0.7%). Compared to control samples, the probiotic E. faecium strain significantly (p < or = 0.05) decreased the amount of total Enterococcus spp. and E. faecalis cells in the colon of 14 day old suckling piglets as well as in jejunum and colon samples one week after weaning. E. faecium cell counts were not modified on any sampling day or intestinal segment. This study showed that the presence of probiotic E. faecium NCIMB10415 coincided with reduced total E. faecalis, but not total E. faecium cell numbers in the intestine of piglets. In view of unchanged cell numbers and ratios in sow feces, modifications must have taken place within the intestine of suckling piglets.
给母猪及其仔猪喂食添加或不添加益生菌屎肠球菌NCIMB10415（也称为SF68）的日粮。仔猪在出生后14、28、35和56天被宰杀，提取并纯化肠道段的DNA。使用实时PCR测定法区分肠球菌属（基于16s rDNA）、屎肠球菌（Efaafm基因）、粪肠球菌（Efaafs基因）以及益生菌菌株（独特的质粒序列）。用接种屎肠球菌和粪肠球菌培养物的高压灭菌母猪粪便提取物校准实时PCR结果。在14日龄的哺乳仔猪能够接触到开食料之前就检测到了益生菌菌株。在益生菌组的仔猪中，益生菌屎肠球菌细胞计数在胃消化物中始终占屎肠球菌细胞总数的显著比例（4 - 20%），然而在所有肠道段中，第14天和28天时，仅占肠球菌属细胞总数的一小部分（0.1 - 0.7%）。与对照样品相比，益生菌屎肠球菌菌株显著（p≤0.05）减少了14日龄哺乳仔猪结肠以及断奶一周后空肠和结肠样品中肠球菌属和粪肠球菌细胞的数量。在任何采样日或肠道段，屎肠球菌细胞计数均未改变。这项研究表明，益生菌屎肠球菌NCIMB１０４１５的存在与仔猪肠道中粪肠球菌总数的减少相吻合，但屎肠球菌总数未减少。鉴于母猪粪便中细胞数量和比例未变，这种变化肯定发生在哺乳仔猪的肠道内。
