Finger Werner J, Shao Benqi, Hoffmann Marcus, Kanehira Masafumi, Endo Tasuo, Komatsu Masashi
Department of Preclinical Dentistry, School of Dental Medicine, University of Cologne, Cologne, Germany.
J Adhes Dent. 2007 Apr;9(2):169-73.
To investigate whether application of three all-in-one self-etching adhesives in a phase-separated stage has an adverse effect on bonding to enamel and dentin.
The shear bond strengths (SBSs) on ground enamel and dentin of the adhesives G-Bond, Hybrid Bond, and iBond, combined with Venus composite, were determined. The adhesives were dispensed in a dish and applied either 1) immediately, 2) after 2 min, or 3) after 5 min dark storage under ambient conditions. The adhesives were shortly stirred with a microbrush and applied. Prior to light activation, the adhesives were air dried for 10 s with a strong air blast. Composite cylinders were produced in a cylindrical mold clamped on the treated surface. SBSs (n = 8) were determined in a universal testing machine at 1 mm/min crosshead speed after 24 h storage in 37 degrees C water. Data were statistically analyzed with Kruskal-Wallis ANOVA and the Mann-Whitney U-Test (p < 0.05). The qualitative compositions of the separated phases were determined by FTIR spectroscopy.
In the separated stage, the adhesives showed acidic monomer-rich phases, containing all components of the original adhesive composition, and water-rich phases with minor amounts of mainly functional monomers dissolved. The SBSs of the individual adhesives applied on enamel or dentin at the different times after dispensing were not significantly different.
Application of the adhesives tested either as homogeneous solution or in a phase-separated stage has no adverse effect on bonding strength to enamel or dentin when applied under the present study conditions.
研究三种一体化自酸蚀粘结剂在相分离阶段的应用是否会对与釉质和牙本质的粘结产生不利影响。
测定了粘结剂G-Bond、Hybrid Bond和iBond与Venus复合树脂结合后在磨除的釉质和牙本质上的剪切粘结强度（SBS）。将粘结剂置于培养皿中，分别在以下条件下应用：1）立即应用；2）2分钟后应用；3）在环境条件下黑暗储存5分钟后应用。用微型刷对粘结剂稍作搅拌后应用。在光固化前，用强气流将粘结剂吹干10秒。在夹于处理过表面的圆柱形模具中制作复合树脂圆柱体。在37℃水中储存24小时后，在万能试验机上以1mm/min的十字头速度测定SBS（n = 8）。数据采用Kruskal-Wallis方差分析和Mann-Whitney U检验进行统计学分析（p < 0.05）。通过傅里叶变换红外光谱法测定分离相的定性组成。
在相分离阶段，粘结剂呈现富含酸性单体的相，其中包含原始粘结剂组合物的所有成分，以及富含水的相，其中溶解有少量主要为功能性单体。在不同时间点应用于釉质或牙本质上的各粘结剂的SBS无显著差异。
在本研究条件下应用时，所测试的粘结剂无论是作为均匀溶液还是在相分离阶段应用，对与釉质或牙本质的粘结强度均无不利影响。
