Chen Meihong, Du Quan, Zhang Hong-Yan, Wang Xiaoxia, Liang Zicai
Chinese Human Genome Center Beijing, Institute of Basic Medical Sciences, Beijing, China.
Expert Rev Mol Diagn. 2007 May;7(3):281-91. doi: 10.1586/14737159.7.3.281.
RNA interference (RNAi) has become one of the most important research tools in functional genomics analysis ever since the discovery of the phenomenon. The robustness of the method has enabled construction of RNAi libraries in the forms of long double-stranded RNA or short-interfering RNA that can cover the whole or significant parts of the genomes of different organisms. Over the last few years, such libraries have been used in different high-throughput formats to establish functional links between genes and phenotypes. In this review, available RNAi library resources and application of these strategic tools will be discussed.
自从RNA干扰（RNAi）现象被发现以来，它已成为功能基因组学分析中最重要的研究工具之一。该方法的稳健性使得能够构建长双链RNA或短干扰RNA形式的RNAi文库，这些文库可以覆盖不同生物体基因组的全部或大部分。在过去几年中，此类文库已被用于不同的高通量形式，以建立基因与表型之间的功能联系。在这篇综述中，将讨论现有的RNAi文库资源以及这些策略性工具的应用。
