从偏好Shaker钾通道的以色列金蝎（Buthus occitanus israelis）中分离出第一种毒素。
Isolation of the first toxin from the scorpion Buthus occitanus israelis showing preference for Shaker potassium channels.
作者信息
Kozminsky-Atias Adi, Somech Erez, Zilberberg Noam
机构信息
Department of Life Sciences and the Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
出版信息
FEBS Lett. 2007 May 29;581(13):2478-84. doi: 10.1016/j.febslet.2007.04.065. Epub 2007 Apr 30.
We have purified BoiTx1, the first toxin from the venom of the Israeli scorpion, Buthus occitanus israelis, and studied its activity and genomic organization. BoiTx1 is a 37 amino acid-long peptide contained six conserved cysteines, and is classified as an alpha-KTx3.10 toxin. The pharmacological effects of BoiTx1 were studied on various cloned K(+) channels expressed in Xenopus laevis oocytes. BoiTx1 inhibited currents through Drosophila Shaker channels with an IC(50) value of 3.5+/-0.5nM, yet had much lesser effect on its mammalian orthologs. Thus, BoiTx1 is the first member of the alpha-KTx3 family that preferentially affects insect potassium channels.
我们已经纯化了来自以色列蝎子（Buthus occitanus israelis）毒液的第一种毒素BoiTx1，并研究了其活性和基因组结构。BoiTx1是一种由37个氨基酸组成的肽，含有六个保守的半胱氨酸，被归类为α-KTx3.10毒素。我们研究了BoiTx1对非洲爪蟾卵母细胞中表达的各种克隆K(+)通道的药理作用。BoiTx1抑制通过果蝇Shaker通道的电流，IC(50)值为3.5±0.5nM，但对其哺乳动物同源物的作用要小得多。因此，BoiTx1是α-KTx3家族中第一个优先影响昆虫钾通道的成员。
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