来自伊朗蝎子奥氏钝尾蝎毒液的首个钾通道毒素。
The first potassium channel toxin from the venom of the Iranian scorpion Odonthobuthus doriae.
作者信息
Abdel-Mottaleb Yousra, Clynen Elke, Jalali Amir, Bosmans Frank, Vatanpour Hossein, Schoofs Liliane, Tytgat Jan
机构信息
Laboratory of Toxicology, University of Leuven, Onderwijs and Navorsing II, Herestraat 49, P.O. Box 922, 3000 Leuven, Belgium.
出版信息
FEBS Lett. 2006 Nov 13;580(26):6254-8. doi: 10.1016/j.febslet.2006.10.029. Epub 2006 Oct 20.
The very first member of K(+) channels toxins from the venom of the Iranian scorpion Odonthobuthus doriae (OdK1) was purified, sequenced and characterized physiologically. OdK1 has 29 amino acids, six conserved cysteines and a pI value of 4.95. Based on multiple sequence alignments, OdK1 was classified as alpha-KTx 8.5. The pharmacological effects of OdK1 were studied on six different cloned K(+) channels (vertebrate Kv1.1-Kv1.5 and Shaker IR) expressed in Xenopus laevis oocytes. Interestingly, OdK1 selectively inhibited the currents through Kv1.2 channels with an IC50 value of 183+/-3 nM but did not affect any of the other channels.
来自伊朗蝎子奥氏真钳蝎（OdK1）毒液的钾离子通道毒素的首个成员被纯化、测序并进行了生理特性鉴定。OdK1有29个氨基酸、6个保守的半胱氨酸，其等电点为4.95。基于多序列比对，OdK1被归类为α-KTx 8.5。研究了OdK1对非洲爪蟾卵母细胞中表达的六种不同克隆钾离子通道（脊椎动物Kv1.1-Kv1.5和Shaker IR）的药理作用。有趣的是，OdK1以183±3 nM的半数抑制浓度（IC50）选择性抑制通过Kv1.2通道的电流，但不影响其他任何通道。
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