Mustafa A M, Malintan N T, Seelan S, Zhan Z, Mohamed Z, Hassan J, Pendek R, Hussain R, Ito N
Department of Pharmacology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Toxicol Appl Pharmacol. 2007 Jul 1;222(1):25-32. doi: 10.1016/j.taap.2007.03.014. Epub 2007 Mar 24.
This study is a result of an analysis of free and conjugated phytoestrogens daidzein, genistein, daidzin, genistin and coumesterol in human cord blood plasma using LCMS. Cord blood was collected from urban and rural populations of Malaysia (n=300) to establish a simple preliminary database on the levels of the analyzed compounds in the collected samples. The study also aimed to look at the levels of phytoestrogens in babies during birth as this may have a profound effect on the developmental process. The sample clean up was carried out by solid-phase extraction using C18 column and passed through DEAE sephadex gel before analysis by LCMS. The mean concentrations of total phytoestrogens were daidzein (1.4+/-2.9 ng/ml), genistein (3.7+/-2.8 ng/ml), daidzin (3.5+/-3.1 ng/ml), genistin (19.5+/-4.2 ng/ml) and coumesterol (3.3+/-3.3 ng/ml). Distribution of phytoestrogen was found to be higher in samples collected from rural areas compared to that of urban areas.
本研究是使用液相色谱 - 质谱联用仪（LCMS）对人脐带血血浆中游离和结合型植物雌激素大豆苷元、染料木黄酮、大豆苷、染料木苷和香豆雌酚进行分析的结果。从马来西亚城市和农村人口中收集脐带血（n = 300），以建立所采集样本中分析化合物水平的简单初步数据库。该研究还旨在观察婴儿出生时植物雌激素的水平，因为这可能对发育过程产生深远影响。样本净化通过使用C18柱的固相萃取进行，并在通过LCMS分析之前通过二乙氨基乙基葡聚糖凝胶。总植物雌激素的平均浓度为大豆苷元（1.4±2.9纳克/毫升）、染料木黄酮（3.7±2.8纳克/毫升）、大豆苷（3.5±3.1纳克/毫升）、染料木苷（19.5±4.2纳克/毫升）和香豆雌酚（3.3±3.3纳克/毫升）。发现从农村地区采集的样本中植物雌激素的分布高于城市地区。
