Vijayaraghavan K, Han Min Hee, Choi Sun Beom, Yun Yeoung-Sang
Division of Environmental and Chemical Engineering, Research Institute of Industrial Technology, Chonbuk National University, Chonju 561-756, South Korea.
Chemosphere. 2007 Aug;68(10):1838-45. doi: 10.1016/j.chemosphere.2007.03.030. Epub 2007 May 8.
Corynebacterium glutamicum, a lysine fermentation industry waste, showed promise for the removal of Reactive black 5 (RB5). Due to practical difficulties in solid-liquid separation, the free biomass was immobilized in two polymer matrices: calcium alginate and polysulfone. Initially, the optimization of biomass loading in polymeric beads and bead dosage were examined. Of the different combinations examined, 4% (with bead dosage of 2 g per 40 ml) and 14% (with bead dosage of 1 g per 40 ml) in the case of alginate and polysulfone beads, respectively, were identified as the optimal conditions. According to the Langmuir model, at pH 1, the maximum RB5 uptakes of 352, 282 and 291 mg g(-1) were observed for free, alginate and polysulfone-immobilized biomass, respectively. According to the Weber-Morris model, intraparticle diffusion was found to be the potential rate limiting step for the immobilized beads. Regeneration experiments, with 0.01 M NaOH and Na(2)CO(3) as eluents, revealed that polysulfone beads exhibited invariable RB5 uptake capacity and very high mechanical stability even at the end of twentieth cycle, confirming the technical feasibility of the biosorption process for industrial applications.
谷氨酸棒杆菌是赖氨酸发酵工业的废弃物，在去除活性黑5（RB5）方面显示出潜力。由于固液分离存在实际困难，将游离生物质固定在两种聚合物基质中：海藻酸钙和聚砜。最初，研究了聚合物珠粒中生物质负载量和珠粒剂量的优化。在所研究的不同组合中，海藻酸钙珠粒和聚砜珠粒分别为4%（每40毫升2克珠粒剂量）和14%（每40毫升1克珠粒剂量）被确定为最佳条件。根据朗缪尔模型，在pH值为1时，游离、海藻酸钙固定化和聚砜固定化生物质对RB5的最大吸附量分别为352、282和291毫克/克。根据韦伯-莫里斯模型，发现颗粒内扩散是固定化珠粒潜在的限速步骤。以0.01 M氢氧化钠和碳酸钠作为洗脱剂的再生实验表明，即使在第二十次循环结束时，聚砜珠粒仍表现出不变的RB5吸附能力和非常高的机械稳定性，证实了生物吸附过程在工业应用中的技术可行性。
