Yamanoue Yusuke, Miya Masaki, Matsuura Keiichi, Yagishita Naoki, Mabuchi Kohji, Sakai Harumi, Katoh Masaya, Nishida Mutsumi
Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano-ku, Tokyo 164-8639, Japan.
Mol Phylogenet Evol. 2007 Oct;45(1):89-101. doi: 10.1016/j.ympev.2007.03.008. Epub 2007 Mar 27.
Tetraodontiformes includes approximately 350 species assigned to nine families, sharing several reduced morphological features of higher teleosts. The order has been accepted as a monophyletic group by many authors, although several alternative hypotheses exist regarding its phylogenetic position within the higher teleosts. To date, acanthuroids, zeiforms, and lophiiforms have been proposed as sister-groups of the tetraodontiforms. The monophyly and sister-group status was investigated using whole mitochondrial genome (mitogenome) sequences from 44 purposefully-chosen species (26 sequences newly-determined during the study) that fully represent the major tetraodontiform lineages plus all the groups that have been hypothesized as being close relatives. Partitioned Bayesian analyses were conducted with the three datasets that comprised concatenated nucleotide sequences from 13 protein-coding genes (with and without, or with RY-coding, 3rd codon positions), plus 22 transfer RNA and two ribosomal RNA genes. The resultant trees were well resolved and largely congruent, with most internal branches being supported by high posterior probabilities. Mitogenomic data strongly supported the monophyly of tetraodontiform fishes, placing them as a sister-group of either Lophiiformes plus Caproidei or Caproidei only. The sister-group relationship between Acanthuroidei and Tetraodontiformes was statistically rejected using Bayes factors. These results were confirmed by a reanalysis of the previously published nuclear RAG1 gene sequences using the Bayesian method. Within the Tetraodontiformes, however, monophylies of the three superfamilies were not recovered and further taxonomic sampling and subsequent efforts should clarify these relationships.
鲀形目包括约350个物种，分为9个科，具有一些高等硬骨鱼简化的形态特征。尽管关于其在高等硬骨鱼中的系统发育位置存在几种不同的假说，但许多作者已将该目视为一个单系类群。迄今为止，刺尾鱼目、海鲂目和鮟鱇目已被提议作为鲀形目的姐妹类群。利用从44个特意挑选的物种（研究期间新测定了26个序列）获得的全线粒体基因组（线粒体基因组）序列对单系性和姐妹类群地位进行了研究，这些物种充分代表了主要的鲀形目谱系以及所有被假设为近亲的类群。使用三个数据集进行分区贝叶斯分析，这三个数据集包括来自13个蛋白质编码基因（有或无，或采用RY编码，第三密码子位置）的串联核苷酸序列，加上22个转运RNA和两个核糖体RNA基因。得到的树解析良好且基本一致，大多数内部分支得到高后验概率的支持。线粒体基因组数据有力地支持了鲀形目鱼类的单系性，将它们置于鮟鱇目加须鳂科或仅须鳂科的姐妹类群位置。使用贝叶斯因子从统计学上否定了刺尾鱼亚目与鲀形目之间的姐妹类群关系。通过使用贝叶斯方法对先前发表的核RAG1基因序列进行重新分析，证实了这些结果。然而，在鲀形目内部，三个超科的单系性并未得到恢复，进一步的分类取样及后续工作应能阐明这些关系。
