Kern Jan, Biesiadka Jacek, Loll Bernhard, Saenger Wolfram, Zouni Athina
Institut für Chemie, Max Volmer Laboratorium für Biophysikalische Chemie, Sekr. PC 14, Technische Universität Berlin, Strasse des 17. Juni 135, 10623 Berlin, Germany.
Photosynth Res. 2007 Jun;92(3):389-405. doi: 10.1007/s11120-007-9173-1. Epub 2007 May 11.
The catalytic centre for light-induced water oxidation in photosystem II (PSII) is a multinuclear metal cluster containing four manganese and one calcium cations. Knowing the structure of this biological catalyst is of utmost importance for unravelling the mechanism of water oxidation in photosynthesis. In this review we describe the current state of the X-ray structure determination at 3.0 A resolution of the water oxidation complex (WOC) of PSII. The arrangement of metal cations in the cluster, their coordination and protein surroundings are discussed with regard to spectroscopic and mutagenesis studies. Limitations of the presently available structural data are pointed out and possible perspectives for the future are outlined, including the combination of X-ray diffraction and X-ray spectroscopy on single crystals.
光系统II（PSII）中光诱导水氧化的催化中心是一个包含四个锰阳离子和一个钙阳离子的多核金属簇。了解这种生物催化剂的结构对于阐明光合作用中水氧化的机制至关重要。在这篇综述中，我们描述了PSII水氧化复合物（WOC）在3.0埃分辨率下X射线结构测定的当前状态。结合光谱学和诱变研究，讨论了簇中金属阳离子的排列、它们的配位情况以及蛋白质环境。指出了目前可用结构数据的局限性，并概述了未来可能的前景，包括对单晶进行X射线衍射和X射线光谱学的结合。
