Goll Rasmus, Gruber Franz, Olsen Trine, Cui Guanglin, Raschpichler Gabriele, Buset Magne, Asfeldt Anne M, Husebekk Anne, Florholmen Jon
Institute of Clinical Medicine, Medical Faculty, University of Tromsø, Norway.
Helicobacter. 2007 Jun;12(3):185-92. doi: 10.1111/j.1523-5378.2007.00495.x.
Host factors play an important role in the pathophysiology of Helicobacter pylori infection and development of gastritis and related disease. The established opinion is that the T-cell-mediated immune response to H. pylori infection is of Th1 type. Our earlier immune cell phenotype studies indicate a mixed Th1-Th2 profile of the effector cells. Therefore, an extensive adaptive and regulatory cytokine gene expression profile was conducted by quantitative real-time polymerase chain reaction (qPCR).
Biopsies from gastric mucosa of 91 patients diagnosed as H. pylori negative, H. pylori positive with gastritis, or H. pylori positive with peptic ulcer were obtained by endoscopy. Gene expressions of nine cytokines and CagA status were measured by qPCR.
All cytokine genes showed higher expression levels in the presence of H. pylori when compared to H. pylori-negative samples (fold increase: IL8: x 11.2; IL12A: x 2.4; TNF-alpha: x 5.2; IFN-gamma: x 4.3; IL4: x 3.6; IL6: x 14.7; and IL10: x 6.7). Patients infected with CagA-positive strains had higher expression of IL1-beta and IL18 compared to patients infected with CagA-negative strains (x 1.6 for IL1-beta and x 2.0 for IL18). Patients with duodenal ulcer had a lower antral Th1/Th2 ratio than other H. pylori-positive patients.
The cytokine profile of H. pylori-infected gastric mucosa shows a mixed Th1-Th2 profile. Furthermore, a high IL10 expression may indicate that also regulatory T cells play a role in the chronic phase of H. pylori infection.
宿主因素在幽门螺杆菌感染、胃炎及相关疾病的病理生理学中起重要作用。既定观点认为，针对幽门螺杆菌感染的T细胞介导免疫反应为Th1型。我们早期的免疫细胞表型研究表明效应细胞呈现Th1-Th2混合特征。因此，通过定量实时聚合酶链反应（qPCR）对广泛的适应性和调节性细胞因子基因表达谱进行了研究。
通过内镜检查从91例诊断为幽门螺杆菌阴性、幽门螺杆菌阳性伴胃炎或幽门螺杆菌阳性伴消化性溃疡的患者胃黏膜获取活检组织。通过qPCR测量9种细胞因子的基因表达及CagA状态。
与幽门螺杆菌阴性样本相比，所有细胞因子基因在存在幽门螺杆菌时均显示出更高的表达水平（倍数增加：IL8：11.2倍；IL12A：2.4倍；TNF-α：5.2倍；IFN-γ：4.3倍；IL4：3.6倍；IL6：14.7倍；IL10：6.7倍）。与感染CagA阴性菌株的患者相比，感染CagA阳性菌株的患者IL1-β和IL18表达更高（IL1-β为1.6倍，IL18为2.0倍）。十二指肠溃疡患者胃窦部的Th1/Th2比值低于其他幽门螺杆菌阳性患者。
幽门螺杆菌感染的胃黏膜细胞因子谱呈现Th1-Th2混合特征。此外，高IL10表达可能表明调节性T细胞在幽门螺杆菌感染的慢性期也发挥作用。
