Milic Ljiljana, Karamarkovic Aleksandar, Popadic Dusan, Sijacki Ana, Grigorov Ilijana, Milosevic Emina, Cuk Vladica, Pesko Predrag
Surgical Clinic "Nikola Spasić", Zvezdara University Medical Center, Faculty of Medicine, University Belgrade, Dimitrija Tucovića 161, 11000, Belgrade, Serbia.
Institute of Microbiology and Immunology, Faculty of Medicine, University Belgrade, Dr Subotica 1, 11000, Belgrade, Serbia.
BMC Res Notes. 2019 May 15;12(1):278. doi: 10.1186/s13104-019-4310-4.
Peptic ulcer disease is a condition in which an important role has infection with H. pylori. The most common complication of peptic ulcer is bleeding. The presence of H. pylori triggers local and systemic cytokine signaling which may affect processes such as healing, gastric or duodenal rupture, and carcinogenesis. In this study, we examined the concentrations of IL-1β, IL-6, IL-10, TNF, TGF-β and IL-17A in serum by enzyme immunoassay and their mRNA expressions in periulcer biopsies obtained from patients with bleeding peptic ulcer by means of real-time-PCR.
We have shown that pro-inflammatory IL-6 and TNF concentrations in serum were significantly higher in patients who were infected with H. pylori, while the concentrations of TGF-β and IL-17A were significantly lower compared to non-infected subjects. IL-17A expression in periulcer mucosa was significantly higher in patients who were infected with H. pylori, while the expression of other cytokines, there was no significant difference compared to non-infected controls. Considering higher serum concentrations in non-infected subjects and higher IL-17A expression in mucosal tissue of infected patients, our data support the studies that found IL-17A has protective role in eradication of H. pylori infection in infected patients.
消化性溃疡病中幽门螺杆菌感染起着重要作用。消化性溃疡最常见的并发症是出血。幽门螺杆菌的存在会触发局部和全身细胞因子信号传导，这可能会影响诸如愈合、胃或十二指肠破裂以及致癌作用等过程。在本研究中，我们通过酶免疫测定法检测了血清中白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏死因子（TNF）、转化生长因子-β（TGF-β）和白细胞介素-17A（IL-17A）的浓度，并通过实时聚合酶链反应检测了从出血性消化性溃疡患者获取的溃疡周围活检组织中它们的mRNA表达。
我们发现，幽门螺杆菌感染患者血清中促炎细胞因子IL-6和TNF的浓度显著高于未感染患者，而TGF-β和IL-17A的浓度则显著低于未感染患者。幽门螺杆菌感染患者溃疡周围黏膜中IL-17A的表达显著高于未感染患者，而其他细胞因子的表达与未感染对照组相比无显著差异。考虑到未感染患者血清浓度较高以及感染患者黏膜组织中IL-17A表达较高，我们的数据支持了那些发现IL-17A在感染患者根除幽门螺杆菌感染中具有保护作用的研究。
