He Mi-Chun, Li Jing, Zhao Chun-Hua
Center of Tissue Engineering, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing 100005, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007 Apr;15(2):433-6.
Oxygen is essential for life, but cultivation of cells is usually performed under 20% O(2), that do not replicate normal physiological hypoxia or pathological hypoxia conditions in the body. Recently, the effect of hypoxia on mesenchymal stem cells (MSCs) has been studied, under physiological hypoxia, MSCs thrive well, and the ability differentiating to osteoblast, chondrocyte and adipocyte as well as the ability of migration are changed. Hypoxia changes the physiological characteristics of embryonic stem cell, hematopoietic stem cell and neuron stem cell as well. The mechanism of these responses might be primarily involved in the hypoxic inducible factor-1 (HIF-1) signal pathway. This review emphasizes that hypoxia is an important factor on all major aspects of stem cell biology including survival, proliferation, differentiation, and migration, and the mechanism involved in HIF-1 signaling pathway behind these responses was also discussed.
氧气对生命至关重要，但细胞培养通常在20%氧气条件下进行，这无法模拟体内正常的生理缺氧或病理缺氧状态。最近，人们研究了缺氧对间充质干细胞（MSC）的影响，在生理缺氧条件下，MSC生长良好，其向成骨细胞、软骨细胞和脂肪细胞分化的能力以及迁移能力均发生改变。缺氧也会改变胚胎干细胞、造血干细胞和神经干细胞的生理特性。这些反应的机制可能主要涉及缺氧诱导因子-1（HIF-1）信号通路。本综述强调，缺氧是干细胞生物学所有主要方面（包括存活、增殖、分化和迁移）的一个重要因素，同时还讨论了这些反应背后HIF-1信号通路所涉及的机制。
