Miki Toshio, Mitamura Keitaro, Ross Mark A, Stolz Donna B, Strom Stephen C
Department of Pathology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
J Reprod Immunol. 2007 Oct;75(2):91-6. doi: 10.1016/j.jri.2007.03.017. Epub 2007 May 9.
The placenta contains different populations of stem/progenitor cells such as mesenchymal, hematopoietic, trophoblastic and pluripotent stem cells. Although some tissue-specific stem cells are restricted to particular parts of the placenta, the localization of embryonic stem cell-like cells in term human placenta has not been determined. We have used immunofluorescence staining techniques with antibodies to pluripotent stem cell antigens, SSEA-3, SSEA-4, TRA 1-60 and TRA 1-81, and confocal microscopic analysis to identify and localize stem cells within the placenta. Stem cell marker-positive cells were found in amnion but not in choriodecidua, tissues known to contain hematopoietic and trophoblastic stem cells. Amniotic mesenchymal cells did not react with these pluripotent stem cell markers, while all amniotic epithelial cells reacted with at least one antibody. The TRA 1-60 and TRA 1-81 positive cells were solitary and present throughout the surface of amniotic membrane without a specific pattern of distribution, whereas SSEA-3 was negative and SSEA-4 was weakly positive on all amniotic epithelial cells. These data suggest that the human amnion contains stem cell-like cells at different states of differentiation. Human term amnion may be useful source of pluripotent stem cells for regenerative medicine.
胎盘包含不同群体的干细胞/祖细胞，如间充质干细胞、造血干细胞、滋养层干细胞和多能干细胞。尽管一些组织特异性干细胞局限于胎盘的特定部位，但足月人胎盘中类胚胎干细胞的定位尚未确定。我们使用针对多能干细胞抗原SSEA - 3、SSEA - 4、TRA 1 - 60和TRA 1 - 81的抗体进行免疫荧光染色技术，并通过共聚焦显微镜分析来识别和定位胎盘内的干细胞。在羊膜中发现了干细胞标志物阳性细胞，但在已知含有造血干细胞和滋养层干细胞的绒毛膜蜕膜中未发现。羊膜间充质细胞不与这些多能干细胞标志物反应，而所有羊膜上皮细胞至少与一种抗体反应。TRA 1 - 60和TRA 1 - 81阳性细胞呈孤立状态，分布于整个羊膜表面，无特定分布模式，而所有羊膜上皮细胞上SSEA - 3呈阴性，SSEA - 4呈弱阳性。这些数据表明，人羊膜含有处于不同分化状态的类干细胞。足月人羊膜可能是再生医学中多能干细胞的有用来源。
