Lepère Charlotte, Régeard Morgane, Le Seyec Jacques, Gripon Philippe
INSERM U522, Hôpital de Pontchaillou, Avenue Henri le Guilloux, Rennes Cedex 35033, France.
J Virol. 2007 Jul;81(14):7816-8. doi: 10.1128/JVI.00224-07. Epub 2007 May 9.
The early events of hepatitis B virus (HBV) infection remain unclear. In 2006, Stoeckl et al. proposed a new entry mechanism involving a translocation motif (TLM) present in the pre-S2 domain of envelope proteins (L. Stoeckl, A. Funk, A. Kopitzki, B. Brandenburg, S. Oess, H. Will, H. Sirma, and E. Hildt, Proc. Natl. Acad. Sci. USA 103:6730-6734, 2006). After receptor binding and internalization into the endosomal compartment, this motif would allow the translocation of HBV particles through the endosomal membrane into the cytosol. In this study we have used two different mutated viruses containing a truncated TLM and showed their ability to infect human hepatocytes in primary culture, thus demonstrating the dispensability of the TLM for HBV infectivity.
乙型肝炎病毒（HBV）感染的早期事件仍不清楚。2006年，施托克等人提出了一种新的进入机制，该机制涉及包膜蛋白前S2结构域中存在的易位基序（TLM）（L.施托克、A.芬克、A.科比茨基、B.勃兰登堡、S.厄斯、H.威尔、H.西尔马和E.希尔特，《美国国家科学院院刊》103:6730 - 6734，2006年）。在与受体结合并内化到内体区室后，该基序将允许HBV颗粒通过内体膜转运到细胞质中。在本研究中，我们使用了两种含有截短TLM的不同突变病毒，并展示了它们在原代培养中感染人肝细胞的能力，从而证明了TLM对HBV感染性并非必需。
