Burova L M, Gorb T E, Tovkach F I
Mikrobiol Z. 2007 Mar-Apr;69(2):23-8.
High specificity of Tn9 transposition into the cryptic plasmid pCA25 of Erwinia carotovora subsp. carotovora 48A was established on the basis of the comparative restriction analysis of DNA by endonucleases EcoRV and PstI. Under the extremal conditions of growth (1% SDS) the plasmid pCA25:: Tn9 loses about 50 % of transpozone sequence, however the cells are not healed of extrachromosomal DNA. The inheritance stability, side by side with site-specific transposition and deleting of Tn9, confirms a supposition on the prophage origin of extrachromosomal element pCA25 of E. carotovora subsp. carotovora 48A.
基于用EcoRV和PstI核酸内切酶对DNA进行的比较限制性分析，确定了Tn9转座到胡萝卜软腐欧文氏菌胡萝卜软腐亚种48A的隐蔽质粒pCA25中的高特异性。在极端生长条件下（1%十二烷基硫酸钠），质粒pCA25::Tn9失去约50%的转座区序列，然而细胞并未消除染色体外DNA。Tn9的位点特异性转座和缺失与遗传稳定性并存，证实了关于胡萝卜软腐欧文氏菌胡萝卜软腐亚种48A染色体外元件pCA25的前噬菌体起源的推测。
