Nikolaĭchik E A, Pesniakevich A G
Genetika. 1995 Aug;31(8):1052-8.
A circular genetic map of the bacterium Erwinia carotovora subsp. atroseptica 3-2 was constructed on the basis of the R471a plasmid and Tn5 and Tn9 using Hfr-like donors. Forty-six genes, including phytopathogenicity genes, were located on the basis of interrupted mating experiment results and analysis of coinheritance of markers on a map of 183 min in length. The similarity and differences of chromosomal genetic maps of Erwinia genus bacteria are discussed.
基于R471a质粒以及Tn5和Tn9,利用类Hfr供体构建了胡萝卜软腐欧文氏菌黑腐亚种3-2的环状遗传图谱。根据中断杂交实验结果以及对长度为183分钟图谱上标记共遗传情况的分析,定位了包括植物致病性基因在内的46个基因。文中还讨论了欧文氏菌属细菌染色体遗传图谱的异同。