Gorb T E, Kushkina A I, Ivanitsa T V, Lysenko T G, Tovkach F I
Mikrobiol Z. 2011 Mar-Apr;73(2):53-7.
Mutants of Erwinia carotovora subsp. carotovra 48A carrying plasmid pCA25 and its transposon variant and resistant to mitomycin C, nalidixic acid and streptomycin were used in the research. It has been shown that the presence of transposon in plasmid pCA25 (strain 48A-7/4b[pCA25::Tn9]) does not practically affect the frequency of appearance of the stable bacterial mutants under the effect of all three antibiotics. Several variants, the plasmid profile of which differs from the initial one were found only in the case of this strain clones resistant to mitomycin C. Electrophoretic mobility of the plasmid DNA of the mutant clones 31 and 32 decreased and approached mobility of the initial plasmid pCA25, the mutant plasmid 13 had high electrophoretic mobility compared to pCA25::Tn9. Some plasmids are the deletion variants of pCA25::Tn9, one of two IS1-elements of the transposon Tn9 being absent in them. The obtained results indicate that the studied plasmid pCA25 is stable, is not eliminated from the cells under different treatments and confirm once more the hypothesis of the prophage origin of the plasmid. The paper is presented in Russian.
研究使用了携带质粒pCA25及其转座子变体且对丝裂霉素C、萘啶酸和链霉素具有抗性的胡萝卜软腐欧文氏菌胡萝卜软腐亚种48A突变体。结果表明，质粒pCA25（菌株48A - 7/4b[pCA25::Tn9]）中的转座子实际上并不影响在所有三种抗生素作用下稳定细菌突变体的出现频率。仅在该菌株对丝裂霉素C有抗性的克隆中发现了几种质粒图谱与初始图谱不同的变体。突变克隆31和32的质粒DNA的电泳迁移率降低，接近初始质粒pCA25的迁移率，突变质粒13与pCA25::Tn9相比具有较高的电泳迁移率。一些质粒是pCA25::Tn9的缺失变体，转座子Tn9的两个IS1元件之一在其中不存在。所得结果表明，所研究的质粒pCA25是稳定的，在不同处理下不会从细胞中消除，并再次证实了该质粒原噬菌体起源的假设。本文用俄语发表。
