Nili H, Asasi K, Dadras H, Ebrahimi S M
Department of Avian Medicine, School of Veterinary Medicine, Shiraz University, P.O. Box 1731, Shiraz 71345, Iran.
Avian Dis. 2007 Mar;51(1 Suppl):390-2. doi: 10.1637/7550-033106R1.1.
Clinical signs, serologic response, viral contents of the trachea and intestine, and histopathological and ultrastructural changes of the tracheal epithelium of Japanese quail experimentally infected with field isolate of H9N2 avian influenza were studied. Vaccinated and unvaccinated quail were inoculated with 10(6.3) 50% embryo infectious dose/bird of A/ chicken/Iran/SH-110/99 (H9N2) virus via nasal inoculation. Clinical signs such as depression, ruffled feathers, diarrhea, and nasal and eye discharges were observed 6 days postinfection (PI). No mortality was observed; however, there was reduction in feed and water consumption and egg production. However, the serologic response of vaccinated challenged and unvaccinated challenged birds was not significantly different. Unvaccinated challenged quail showed more severe histopathologic reaction in their lungs and trachea. Hyperemia, edema, infiltration of inflammatory cells, and deciliation and sloughing of the tracheal epithelium were observed. Ultrastructural study showed dilatation of endoplasmic reticulum and degeneration of Golgi apparatus and cilia of the tracheal lining cells of respiratory epithelium.
研究了经H9N2禽流感野毒株实验感染的日本鹌鹑的临床症状、血清学反应、气管和肠道的病毒含量以及气管上皮的组织病理学和超微结构变化。将接种疫苗和未接种疫苗的鹌鹑通过鼻腔接种10(6.3) 50%胚感染剂量/只的A/鸡/伊朗/SH-110/99 (H9N2)病毒。感染后6天观察到抑郁、羽毛蓬松、腹泻以及鼻和眼分泌物等临床症状。未观察到死亡；然而，采食量、饮水量和产蛋量有所下降。但是，接种疫苗后受攻击和未接种疫苗受攻击的鸟类的血清学反应没有显著差异。未接种疫苗受攻击的鹌鹑在其肺和气管中表现出更严重的组织病理学反应。观察到充血、水肿、炎性细胞浸润以及气管上皮的脱纤毛和脱落。超微结构研究显示内质网扩张以及呼吸上皮气管内衬细胞的高尔基体和纤毛变性。
