Begum Y A, Talukder K A, Nair G B, Khan S I, Svennerholm A-M, Sack R B, Qadri F
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
Can J Microbiol. 2007 Jan;53(1):19-26. doi: 10.1139/w06-098.
Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children. Although the prevalence of ETEC is high in Bangladesh and infections can be spread through food and contaminated water, limited information is available about ETEC in the surface water. We carried out studies to isolate ETEC from surface water samples from ponds, rivers, and a lake from a site close to field areas known to have a high incidence of diarrhea in Dhaka, Bangladesh, and Matlab, Bangladesh. ETEC strains isolated from the water sources were compared with ETEC strains isolated from patients with diarrhea at two hospitals in these areas. ETEC were isolated from 30% (45 of 150) of the samples from the surface water sources and 19% (518 of 2700) of the clinical specimens. One hundred ETEC strains isolated from patients with similar phenotypes as the environmental strains were compared for phenotypic and genotypic properties. The most common O serogroups on ETEC were O6, O25, O78, O115, and O126 in both types of strains. Pulsed-field gel electrophoresis analyses of the ETEC strains showed that multiple clones of ETEC were present within each colonization factor type and that some clones detected in the environment were also isolated from the stools of patients. The strains showed multiple and similar antibiotic resistance patterns. This study shows that ETEC is prevalent in surface water sources in Bangladesh suggesting a possible reason for the endemicity of this pathogen in Bangladesh.
产肠毒素大肠杆菌（ETEC）是导致婴幼儿急性水样腹泻的常见细菌感染病因。尽管孟加拉国ETEC的流行率很高，且感染可通过食物和受污染的水传播，但关于地表水ETEC的信息有限。我们开展了研究，从孟加拉国达卡和孟加拉国马特莱靠近已知腹泻高发地区的池塘、河流及湖泊的地表水样本中分离ETEC。将从水源分离出的ETEC菌株与这些地区两家医院腹泻患者分离出的ETEC菌株进行比较。从地表水样本的30%（150份样本中的45份）和临床标本的19%（2700份标本中的518份）中分离出ETEC。比较了从患者中分离出的100株与环境菌株表型相似的ETEC菌株的表型和基因型特征。两种类型菌株中ETEC最常见的O血清群为O6、O25、O78、O115和O126。对ETEC菌株的脉冲场凝胶电泳分析表明，每种定植因子类型中都存在多个ETEC克隆，且在环境中检测到的一些克隆也从患者粪便中分离出来。这些菌株表现出多种且相似的抗生素耐药模式。这项研究表明ETEC在孟加拉国的地表水水源中普遍存在，这提示了该病原体在孟加拉国流行的一个可能原因。
