Zhang Pingbo, Aso Yoichi, Jikuya Hiroyuki, Kusakabe Takahiro, Lee Jae Man, Kawaguchi Yutaka, Yamamoto Kohji, Banno Yutaka, Fujii Hiroshi
Institute of Genetic Resources, Kyushu University, Fukuoka 812-8581, Japan.
J Proteome Res. 2007 Jun;6(6):2295-303. doi: 10.1021/pr070071y. Epub 2007 May 12.
The silkworm is a typical holometabolous insect going through drastic morphological changes upon metamorphosis from larvae to pupae. Comprehensive studies focusing on the changes help elucidate understanding of a biogenic mechanism. Here, we report the initial profile of the intersegmental muscle (ISM) proteins of the silkworm during larval-pupal metamorphosis. In total, 258 protein spots were resolved by two-dimensional gel electrophoresis (2-DE). Fifty-seven larval proteins were identified, where 3 proteins were exclusively detected in larval samples. Fifty-four other proteins were common in pupal samples. Of these, 12 proteins belonging to the contractile apparatus, metabolism, regulation, and signal transduction were altered in their contents during the metamorphosis from larvae to pupae. Three pupa-defective proteins were identified as isoforms of troponin I, followed by an immunoblotting validation. This data will be helpful in understanding the biochemistry of an insect ISM.
家蚕是一种典型的全变态昆虫，从幼虫到蛹的变态过程中会经历剧烈的形态变化。针对这些变化的综合研究有助于阐明一种生物发生机制。在此，我们报告了家蚕幼虫-蛹变态期间节间肌（ISM）蛋白质的初始概况。通过二维凝胶电泳（2-DE）共分离出258个蛋白质斑点。鉴定出57种幼虫蛋白质，其中3种仅在幼虫样品中检测到。另外54种蛋白质在蛹样品中常见。其中，12种属于收缩装置、代谢、调节和信号转导的蛋白质在从幼虫到蛹的变态过程中其含量发生了变化。鉴定出三种蛹缺陷蛋白为肌钙蛋白I的异构体，并通过免疫印迹验证。这些数据将有助于理解昆虫节间肌的生物化学。
