Qin Lvgao, Shi Haifeng, Xia Hengchuan, Chen Liang, Yao Qin, Chen Keping
Institute of Life Sciences, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu Province, People's Republic of China.
Institute of Life Sciences, Jiangsu University, 301 Xuefu Rd., Zhenjiang 212013, Jiangsu Province, People's Republic of China
J Insect Sci. 2014 Jan 1;14. doi: 10.1093/jisesa/ieu088. Print 2014.
Many biological phenotypes of male and female silkworms (Bombyx mori) are quite different, and one of the major differences is the growth rate at various larval stages. Nutrient utilization by midgut varies with sexes. However, the molecular basis of this variation is not clear. To understand the molecular mechanism, comparative proteomic approach was employed to investigate the variation of midgut proteomes between male and female silkworms. Totally, 32 proteins that were grouped into four categories were differentially expressed and subsequently identified by mass spectrometry. Gene ontology analysis revealed that these proteins were attributed with biological functions such as binding, catalytic, and transporter, and these proteins were involved in biological process such as cellular process, localization, and metabolic process. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that these proteins were involved in pathways such as glycolysis, gluconeogenesis, oxidative phosphorylation, and purine metabolism. At transcription level, the expressional variation was confirmed for six identified proteins including muscle glycogen phosphorylase, uridine 5'-monophosphate synthase, cone cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha, ATP synthase, thiol peroxiredoxin, and serpin-2. This study provides useful information for understanding the mechanisms of nutrient absorption and the protein-protein interaction in the silkworm.
家蚕雌雄个体的许多生物学表型存在显著差异，其中一个主要差异体现在不同幼虫阶段的生长速率上。中肠的营养物质利用情况因性别而异。然而，这种差异的分子基础尚不清楚。为了阐明其分子机制，采用比较蛋白质组学方法研究家蚕雌雄个体中肠蛋白质组的差异。总共鉴定出32种差异表达的蛋白质，它们被分为四类，随后通过质谱分析进行了鉴定。基因本体分析表明，这些蛋白质具有结合、催化和转运等生物学功能，并参与细胞过程、定位和代谢过程等生物学进程。京都基因与基因组百科全书通路分析显示，这些蛋白质参与糖酵解、糖异生、氧化磷酸化和嘌呤代谢等通路。在转录水平上，对包括肌肉糖原磷酸化酶、尿苷5'-单磷酸合酶、视锥细胞cGMP特异性3',5'-环磷酸二酯酶α亚基、ATP合酶、硫氧还蛋白和丝氨酸蛋白酶抑制剂-2在内的6种已鉴定蛋白质的表达差异进行了验证。本研究为理解家蚕营养吸收机制和蛋白质-蛋白质相互作用提供了有用信息。
