雷公藤红素抑制全反式维甲酸引起的白血病细胞与内皮细胞间黏附

[Tripterine inhibits all-trans retinoic acid-caused adhesion between leukemia cells and endothelial cells].

作者信息

Xu Li-min, Zhang Deng-hai, Yang Chun-xin, Liu Xu-hui, Uzan Geroges, Qin Wan-zhang

机构信息

Sino-French Clinical Laboratory, Gongli Hospital, Shanghai 200135, China.

出版信息

Zhong Xi Yi Jie He Xue Bao. 2007 May;5(3):282-6. doi: 10.3736/jcim20070311.

DOI:10.3736/jcim20070311

PMID:17498488

Abstract

OBJECTIVE

Increasing of adhesion between leukemia cells and endothelial cells during all-trans retinoic acid (ATRA) treatment plays an important role in retinoic acid syndrome. This work observed the effects of tripterine on this ATRA-caused increasing in adhesion.

METHODS

The effects of tripterine on ATRA-induced expressions of adhesive molecules in acute promyelocytic leukemia cell line NB4 and human umbilical vascular endothelial cells (HUVEC) were detected by flow cytometry. The effects of tripterine on adhesion between ATRA-treated NB4 and HUVEC were determined by adhesive assays.

RESULTS

ATRA caused remarkable elevation of intercellular adhesion molecule-1 (ICAM-1) in NB4 cells, which could be significantly reduced by tripterine (P<0.01). The expressions of E-selectin, vascular cell adhesion molecule-1 (VCAM-1) and ICAM-1 in HUVEC were elevated by conditioned medium from ATRA-induced NB4 (ATRA-NB4-CM) (P<0.01), and inhibited by tripterine with inhibition rates being 25.3%, 42.4% and 61.0% respectively. ATRA increased the adhesion between NB4 and HUVEC, which was reversed completely by tripterine.

CONCLUSION

Tripterine can inhibit ATRA-caused adhesion between leukemia cells and endothelial cells, and it might be a potential agent for treating retinoic acid syndrome.

摘要

目的

全反式维甲酸（ATRA）治疗期间白血病细胞与内皮细胞间黏附增加在维甲酸综合征中起重要作用。本研究观察了雷公藤红素对此种ATRA诱导的黏附增加的影响。

方法

采用流式细胞术检测雷公藤红素对急性早幼粒细胞白血病细胞系NB4及人脐静脉血管内皮细胞（HUVEC）中ATRA诱导的黏附分子表达的影响。通过黏附实验测定雷公藤红素对经ATRA处理的NB4与HUVEC间黏附的影响。

结果

ATRA使NB4细胞中细胞间黏附分子-1（ICAM-1）显著升高，而雷公藤红素可使其显著降低（P<0.01）。ATRA诱导的NB4条件培养基（ATRA-NB4-CM）可使HUVEC中E-选择素、血管细胞黏附分子-1（VCAM-1）及ICAM-1表达升高（P<0.01），而雷公藤红素可抑制其表达，抑制率分别为25.3%、42.4%及61.0%。ATRA增加了NB4与HUVEC间的黏附，而雷公藤红素可完全逆转此作用。