Zhao Sheng, Zhang Qin, Chen Zhihua, Zhao Yixin, Zhong Jincheng
College of Life Science and Technology, Southwest University for Nationalities Chengdu 610041, China.
J Genet Genomics. 2007 Apr;34(4):362-72. doi: 10.1016/S1673-8527(07)60039-3.
Burkholderia mallei is regarded as a potential biological weapon by the Centers for Disease Control and Prevention. In this study, the main factors shaping codon usage in the genome of B. mallei ATCC 23344 were firstly reported. The results showed that the primary trend in codon usage variation in the B. mallei is due to translational selection; while compositional mutation bias is relatively the weaker influence and the hydrophobicity of each protein and gene length are only the minor influences. At the same time, 21 codons defined firstly as 'optimal codons' might provide more useful information for the expression of target genes and development of a vaccine to prevent glanders.
鼻疽伯克霍尔德菌被疾病控制与预防中心视为一种潜在的生物武器。在本研究中，首次报道了塑造鼻疽伯克霍尔德菌ATCC 23344基因组密码子使用情况的主要因素。结果表明，鼻疽伯克霍尔德菌密码子使用变异的主要趋势是由于翻译选择；而组成性突变偏好的影响相对较弱，各蛋白质的疏水性和基因长度只是次要影响因素。同时，首次定义的21个密码子为“最优密码子”，这可能为靶基因的表达以及预防鼻疽疫苗的研发提供更多有用信息。
