导管乳腺癌中的基因组不稳定以及BUB1B和MAD2L1基因表达增加。
Genomic instability and increased expression of BUB1B and MAD2L1 genes in ductal breast carcinoma.
作者信息
Scintu Marina, Vitale Rita, Prencipe Maria, Gallo Antonietta Pia, Bonghi Loriana, Valori Vanna Maria, Maiello Evaristo, Rinaldi Monica, Signori Emanuela, Rabitti Carla, Carella Massimo, Dallapiccola Bruno, Altomare Vittorio, Fazio Vito M, Parrella Paola
机构信息
Laboratory of Oncology, Research Department, IRCCS Casa Sollievo della Sofferenza, Viale Padre Pio, San Giovanni Rotondo (FG) 71013, Italy.
出版信息
Cancer Lett. 2007 Sep 8;254(2):298-307. doi: 10.1016/j.canlet.2007.03.021. Epub 2007 May 10.
In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24-76.78%) than in controls (average: 11.54%, range: 9.91-14.84%) (P<0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34-35.8%) as compared with controls (average: 4.18% range: 3.47-4.81%) (P<0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r=0.722, P=0.018).
在一系列浸润性导管乳腺癌中，我们通过荧光原位杂交研究了染色体和染色体内不稳定性的状况，并测定了有丝分裂纺锤体检查点途径中两个基因BUB1B和MAD2L1的mRNA表达水平。所有乳腺癌肿瘤样本中的染色体不稳定性率（平均：56.86%，范围：36.24 - 76.78%）均高于对照组（平均：11.54%，范围：9.91 - 14.84%）（P<0.0001）。与对照组（平均：4.18%，范围：3.47 - 4.81%）相比，肿瘤中的染色体内不稳定性率也有所升高（平均：18.45%，范围：8.34 - 35.8%）（P<0.0001）。在大多数检测的肿瘤中，BUB1B和MAD2L1转录本均有增加。BUB1B mRNA水平与染色体内不稳定性相关，而MAD2L1水平则不然（r = 0.722，P = 0.018）。
Zotero 插件