Yalçinkaya Seda, Unlüçerçi Yeşim, Uysal Müjdat
Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, Capa, Istanbul, Turkey.
Exp Toxicol Pathol. 2007 Aug;58(6):455-9. doi: 10.1016/j.etp.2007.03.003. Epub 2007 May 11.
The purpose of this study was to investigate whether high methionine (HM) diet may influence the development of ethanol-induced hepatotoxicity and prooxidant-antioxidant balance in the liver. Rats received drinking water containing ethanol (20% v/v) and/or methionine supplemented diet (2% w/w) for 75 days. Although prooxidant-antioxidant balance did not change in the liver of rats in HM group, ethanol treatment was observed to increase plasma transaminase activities, and malondialdehyde (MDA) and protein carbonyl (PC) levels, but not glutathione (GSH), vitamin E and vitamin C levels, and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione transferase (GST) activities in the liver of rats as compared to controls. However, ethanol plus HM diet caused further increases in plasma transaminase activities and hepatic MDA and PC levels. In addition, SOD, GSH-Px and GST activities were observed to decrease, but GSH, vitamin E and vitamin C levels remained unchanged in the liver as compared to ethanol, HM and control groups. Our results show that HM diet may augment hepatotoxicity and oxidative stress in the liver of chronically ethanol-treated rats.
本研究的目的是调查高蛋氨酸（HM）饮食是否会影响乙醇诱导的肝毒性的发展以及肝脏中的促氧化剂-抗氧化剂平衡。大鼠饮用含乙醇（20% v/v）的水和/或补充蛋氨酸的饮食（2% w/w），持续75天。虽然HM组大鼠肝脏中的促氧化剂-抗氧化剂平衡没有变化，但与对照组相比，观察到乙醇处理会增加血浆转氨酶活性、丙二醛（MDA）和蛋白质羰基（PC）水平，但不会增加谷胱甘肽（GSH）、维生素E和维生素C水平，以及大鼠肝脏中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和谷胱甘肽转移酶（GST）活性。然而，乙醇加HM饮食会导致血浆转氨酶活性以及肝脏中MDA和PC水平进一步升高。此外，与乙醇组、HM组和对照组相比，观察到肝脏中的SOD、GSH-Px和GST活性降低，但GSH、维生素E和维生素C水平保持不变。我们的结果表明，HM饮食可能会增强长期乙醇处理大鼠肝脏中的肝毒性和氧化应激。
