Nagafusa T, Hoshino H, Sakurai T, Terakawa S, Nagano A
Department of Orthopaedic Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan.
Cell Biol Int. 2007 Oct;31(10):1150-9. doi: 10.1016/j.cellbi.2007.03.032. Epub 2007 Mar 31.
The functions of filopodia and lamellipodia in mature osteoclasts are not well known in the process of bone resorption. We investigated the function of filopodial/lamellipodial movement in mature osteoclasts by video-enhanced contrast-differential interference contrast (VEC-DIC) microscopy. Mature osteoclasts, which were isolated from Japanese white rabbits, were cultured on calcium phosphate (CP)-coated quartz coverslips to observe filopodial/lamellipodial movement and the formation of CP-free areas precisely. Filopodia broke the CP substrate into pieces and transported them to the cell body by capturing them at the tip. Lamellipodia destroyed the CP substrate, and transported it to the cell body by capturing small particles in a mass. This study suggests two functions of filopodia and lamellipodia in mature osteoclasts, i.e., the mechanical fragmentation of the CP substrate and the transportation of the CP particles to the cell body.
在骨吸收过程中，成熟破骨细胞中丝状伪足和片状伪足的功能尚不明确。我们通过视频增强对比度-微分干涉对比（VEC-DIC）显微镜研究了成熟破骨细胞中丝状伪足/片状伪足运动的功能。从日本白兔分离出的成熟破骨细胞，培养在磷酸钙（CP）包被的石英盖玻片上，以精确观察丝状伪足/片状伪足的运动以及无CP区域的形成。丝状伪足将CP底物破碎成碎片，并通过在尖端捕获它们将其运输到细胞体。片状伪足破坏CP底物，并通过大量捕获小颗粒将其运输到细胞体。本研究表明成熟破骨细胞中丝状伪足和片状伪足具有两种功能，即CP底物的机械破碎以及CP颗粒向细胞体的运输。
