Takano K, Hall K, Fryklund L, Sievertsson H
Horm Metab Res. 1976 Jan;8(1):16-24. doi: 10.1055/s-0028-1093686.
Particulate membranes prepared from a variety of monkey and rat tissues were shown to have specific binding sites for somatomedin A. Binding to talc on the other hand showed no specificity, and the sensitivity was less. The membrane-bound somatomedin A was displaced by somatomedin A in concentrations between 0.01 and 5 U/ml. Of the other hormones tested only insulin in high concentrations could interfere with the binding of labelled somatomedin A to tissue membranes. Membranes prepared from a number of rat and monkey tissues also contained binding sites for insulin. Somatomedin A could interfere with the binding of labelled insulin. No binding was observed for somatomedin B in the tissues studied.
从多种猴和大鼠组织制备的微粒体膜显示出对生长调节素A有特异性结合位点。另一方面，与滑石粉的结合没有特异性，且敏感性较低。膜结合的生长调节素A可被浓度在0.01至5 U/ml之间的生长调节素A取代。在测试的其他激素中，只有高浓度的胰岛素会干扰标记的生长调节素A与组织膜的结合。从一些大鼠和猴组织制备的膜也含有胰岛素的结合位点。生长调节素A可干扰标记胰岛素的结合。在所研究的组织中未观察到生长调节素B的结合。
