Miura Daisaku, Kida Yoshishige, Nojima Hiroshi
Biosafety Research Center for Foods, Drugs and Pesticides, Iwata, Shizuoka 437-1213, Japan.
FEBS Lett. 2007 May 29;581(13):2541-8. doi: 10.1016/j.febslet.2007.04.080. Epub 2007 May 8.
We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in oleamide-like oleic acid for their inhibitory potency on Cx26-mediated GJIC and spontaneous metastasis of BL6 cells. We found that camellia oil, olive oil and cottonseed oil, and their distillate fractions inhibited Cx26-mediated GJIC. We also showed that daily intraperitoneal injection of camellia oil and its distillate fractions more potently inhibited spontaneous lung metastasis of BL6 cells than oleamide. Moreover, a daily oral administration of camellia oil distillate fraction effectively inhibited spontaneous metastasis. Notably, even camellia Tempura-oil, a commercially available food, weakly inhibited the spontaneous metastasis of BL6 cells. Since these oils are used as foods and are quite safe, we propose that they could be used as supplements to protect patients from lung metastasis of melanomas.
我们之前报道过，每天腹腔注射油酰胺可通过阻断连接蛋白26（Cx26）的间隙连接介导的细胞间通讯（GJIC）来微弱抑制BL6细胞的自发转移。在本研究中，我们测试了富含油酰胺样油酸的山茶油、橄榄油和棉籽油对Cx26介导的GJIC以及BL6细胞自发转移的抑制效力。我们发现，山茶油、橄榄油和棉籽油及其馏分均能抑制Cx26介导的GJIC。我们还表明，每天腹腔注射山茶油及其馏分比油酰胺更有效地抑制BL6细胞的自发肺转移。此外，每天口服山茶油馏分可有效抑制自发转移。值得注意的是，即使是市售食品山茶天妇罗油也能微弱抑制BL6细胞的自发转移。由于这些油被用作食品且相当安全，我们建议它们可作为补充剂，以保护患者免受黑色素瘤肺转移的影响。
