Wang Yanfang, Qu Long, Uthe Jolita J, Bearson Shawn M D, Kuhar Daniel, Lunney Joan K, Couture Oliver P, Nettleton Dan, Dekkers Jack C M, Tuggle Christopher K
Department of Animal Science and Center for Integrated Animal Genomics Iowa State University, Ames, IA 50010, USA.
Genomics. 2007 Jul;90(1):72-84. doi: 10.1016/j.ygeno.2007.03.018. Epub 2007 May 17.
To elucidate the host transcriptional response to Salmonella enterica serovar Typhimurium, Affymetrix porcine GeneChip analysis of pig mesenteric lymph nodes was used to identify 848 genes showing differential expression across different times after inoculation or when compared to non-inoculated controls. Annotation analyses showed that a high proportion of these differentially expressed (DE) genes are involved in immune and inflammatory responses. T helper 1, innate/inflammatory, and antigen-processing pathways were induced at 24 h post-inoculation (hpi) and/or 48 hpi, while apoptosis and antigen presentation/dendritic cell function pathways were downregulated at 8 hpi. Cluster analyses revealed that most DE genes annotated as NFkappaB targets were grouped into a specific induced subcluster, while many translation-related DE genes were found in a repressed subcluster. Quantitative polymerase chain reaction analyses confirmed the Affymetrix results, revealing transcriptional induction of NFkappaB target genes at 24 hpi and suppression of the NFkappaB pathway from 24 to 48 hpi. We propose that such NFkappaB suppression in antigen-presenting cells may be the mechanism by which S. Typhimurium eludes a strong inflammatory response to establish a carrier status in pigs.
为阐明宿主对鼠伤寒沙门氏菌的转录反应，利用Affymetrix猪基因芯片对猪肠系膜淋巴结进行分析，以鉴定出848个在接种后不同时间或与未接种对照相比表现出差异表达的基因。注释分析表明，这些差异表达（DE）基因中有很大一部分参与免疫和炎症反应。接种后24小时（hpi）和/或48 hpi时，辅助性T细胞1、固有/炎症和抗原加工途径被诱导，而在8 hpi时，凋亡和抗原呈递/树突状细胞功能途径被下调。聚类分析显示，大多数注释为NFκB靶标的DE基因被归为一个特定的诱导亚群，而许多与翻译相关的DE基因则出现在一个受抑制的亚群中。定量聚合酶链反应分析证实了Affymetrix的结果，揭示了在24 hpi时NFκB靶基因的转录诱导以及在24至48 hpi时NFκB途径的抑制。我们提出，抗原呈递细胞中这种NFκB的抑制可能是鼠伤寒沙门氏菌逃避强烈炎症反应以在猪体内建立携带状态的机制。
