Grupo de Genómica y Mejora Animal, Departamento de Genética, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain.
Comp Immunol Microbiol Infect Dis. 2013 Mar;36(2):149-60. doi: 10.1016/j.cimid.2012.11.003. Epub 2012 Dec 28.
Infections caused by Salmonella enterica serovar Typhimurium (S. typhimurium) cause important economic problems in the swine industry and threaten the integrity of a safe and healthy food supply. Controlling the prevalence of Salmonella in pig production requires a thorough knowledge of the response processes that occurs in the gut associated immune tissues. To explore the in vivo porcine response to S. typhimurium, MLN samples from four control pigs and twelve infected animals at 1, 2 and 6 days post infection (dpi) were collected to quantify the mRNA expression of gene coding for 42 innate immune-related molecules. In addition, the presence of S. typhimurium in MLN was examined and its effect on tissue micro-anatomy. Higher S. typhimurium loads were observed at 2dpi, triggering an innate immune response, marked by a substantial infiltration of phagocytes and up-regulation of pro-inflammatory genes. Such response resulted in a significant decrease in pathogen burden in MLN at 6dpi, although Salmonella could not be completely eliminated from tissue. Furthermore, our results suggest that in porcine infections, S. typhimurium might interferes with dendritic cell-T cell interactions and this strategy could be involved in the conversion of Salmonella infected pigs to a carrier state.
鼠伤寒沙门氏菌（S. typhimurium）引起的感染给养猪业造成了严重的经济问题，并威胁到安全健康食品供应的完整性。控制猪群中沙门氏菌的流行需要深入了解肠道相关免疫组织中发生的反应过程。为了研究猪体内对 S. typhimurium 的反应，我们从 4 头对照猪和 12 头感染动物的肠系膜淋巴结（MLN）样本中收集了 1、2 和 6 天感染后（dpi）的样本，以定量检测编码 42 种固有免疫相关分子的基因的 mRNA 表达。此外，我们还检查了 MLN 中 S. typhimurium 的存在及其对组织微观解剖结构的影响。在 2dpi 时观察到更高的 S. typhimurium 负荷，引发了固有免疫反应，大量吞噬细胞浸润和促炎基因的上调。这种反应导致 MLN 中的病原体负荷在 6dpi 时显著下降，尽管沙门氏菌不能从组织中完全消除。此外，我们的结果表明，在猪感染中，S. typhimurium 可能干扰树突状细胞与 T 细胞的相互作用，这种策略可能参与了沙门氏菌感染猪转变为带菌状态的过程。
