培养的哺乳动物细胞中一种需要不饱和脂肪酸的突变体的分离与鉴定。

Chang T Y, Vagelos P R

Proc Natl Acad Sci U S A. 1976 Jan;73(1):24-8. doi: 10.1073/pnas.73.1.24.

An unsaturated fatty acid-requiring mutant derived from Chinese hamster ovary (CHO) cells has been isolated and characterized. This mutant grows normally when oleate or other unsaturated fatty acids are supplemented in the growth medium. Unlike the wild-type CHO cells, growth stops when medium is deprived of unsaturated fatty acid. Whole cell pulse experiments with [14C]acetate or [14C]stearate indicate that the mutant is defective in unsaturated fatty acid synthesis. Enzyme assays in vitro show that the enzymatic defect of the mutant is localized to the microsomal stearoyl-CoA desaturase.

已分离并鉴定出一种源自中国仓鼠卵巢（CHO）细胞的不饱和脂肪酸需求型突变体。当在生长培养基中添加油酸或其他不饱和脂肪酸时，该突变体能够正常生长。与野生型CHO细胞不同，当培养基中缺乏不饱和脂肪酸时，生长就会停止。用[14C]乙酸盐或[14C]硬脂酸盐进行的全细胞脉冲实验表明，该突变体在不饱和脂肪酸合成方面存在缺陷。体外酶活性测定表明，该突变体的酶缺陷定位于微粒体硬脂酰辅酶A去饱和酶。