Chattopadhyay P, Banerjee S K, Sen K, Chakrabarti P
Can J Microbiol. 1985 Apr;31(4):346-51. doi: 10.1139/m85-066.
The wild-type Aspergillus niger (V35) does not require fatty acids for growth. Four unsaturated fatty acid auxotrophs designated as UFA1, UFA2, UFA3, and UFA4 have been produced from this organism by treating the conidia of the wild-type strain with a mutagen, N-methyl-N'-nitro-N-nitrosoguanidine, followed by isolation on media containing monounsaturated fatty acids and the nonionic detergent, Brij 58. Optimal growth of the mutants comparable with that of the wild type was achieved with medium supplemented with C16 or C18 unsaturated fatty acids containing at least one cis double bond at the delta 9 position. Some other fatty acids (18:1 delta 11 cis and 16:1 delta 9 trans) support growth to some extent. The mutants do not grow at all in the presence of saturated fatty acids. Fatty acid analyses of the mutant, UFA2, grown in the presence of different fatty acid supplements reveal that it may be defective in a desaturase system. Experiments with unlabeled and [1-14C]palmitoyl-CoA have shown that the microsomes of the mutant (UFA2) contain a partially defective delta 9-desaturase system.
野生型黑曲霉（V35）生长不需要脂肪酸。通过用诱变剂N-甲基-N'-硝基-N-亚硝基胍处理野生型菌株的分生孢子，然后在含有单不饱和脂肪酸和非离子洗涤剂Brij 58的培养基上进行分离，从该生物体中产生了四种不饱和脂肪酸营养缺陷型，分别命名为UFA1、UFA2、UFA3和UFA4。在补充有在Δ9位置含有至少一个顺式双键的C16或C18不饱和脂肪酸的培养基上，突变体实现了与野生型相当的最佳生长。一些其他脂肪酸（18:1Δ11顺式和16:1Δ9反式）在一定程度上支持生长。在饱和脂肪酸存在下，突变体根本不生长。对在不同脂肪酸补充剂存在下生长的突变体UFA2进行的脂肪酸分析表明，它可能在去饱和酶系统中存在缺陷。用未标记的和[1-14C]棕榈酰辅酶A进行的实验表明，突变体（UFA2）的微粒体含有部分缺陷的Δ9-去饱和酶系统。
