Rekstin A R, Kiseleva I V, Desheva Iu A, Kats D, Aleksandrova G I, Klimov A I, Rudenko L G
Vopr Virusol. 2007 Mar-Apr;52(2):13-6.
The authors examined a role of some mutated A/Leningrad/134/17/57(H2N2) virus genes in the realization of growth characteristics. The latter of single gene reassortants (SGRs) (PB2, PB1, PA, M, and NS), epidemic virus and attenuation donor were assessed by infecting MDCK cells and hen embryos at a low inoculation index. Viral replication in the hen embryos and cultured tissue was compared at 34 degrees C. The viruses and reassortants tested showed a high growth capacity in the hen embryos (9.5-10.5 Ig TCID50). The growth curves of viruses were studied on the cultured MDCK cells at a low inoculation index indicated that Len/17 and the single gene reassortants M and NS had the highest growth capacity. At the same time the growth of both PB1 and PB2 SGRs was less extensive. The reproduction of PB2 SGR was 100-1000 times less than that of other viruses tested. M, NS, and PA gene mutations did not affect viral growth in hen embryos and cultured tissue while PB2 gene mutation and its constellations with other genes caused a reduction in viral growth in the cultured tissue.
作者研究了一些突变的A/列宁格勒/134/17/57(H2N2)病毒基因在实现生长特性方面的作用。通过以低接种指数感染MDCK细胞和鸡胚，对单基因重配体（SGRs）（PB2、PB1、PA、M和NS）、流行病毒和减毒供体进行了评估。在34摄氏度下比较了病毒在鸡胚和培养组织中的复制情况。所测试的病毒和重配体在鸡胚中显示出较高的生长能力（9.5 - 10.5 Ig TCID50）。在低接种指数下，在培养的MDCK细胞上研究了病毒的生长曲线，结果表明Len/17以及单基因重配体M和NS具有最高的生长能力。同时，PB1和PB2 SGRs的生长范围较小。PB2 SGR的繁殖能力比其他测试病毒低100 - 1000倍。M、NS和PA基因突变不影响病毒在鸡胚和培养组织中的生长，而PB2基因突变及其与其他基因的组合导致培养组织中病毒生长减少。
