Shichino K, Yukimura T, Miura K, Yamamoto K, Okubo M, Shimazu A
Department of Orthopedic Surgery, Osaka City University Medical School, Japan.
Tohoku J Exp Med. 1991 Jun;164(2):145-56. doi: 10.1620/tjem.164.145.
Skeletal muscle blood flow (MBF) and intramuscular pressure (IMP) were measured using a heated thermocouple (HTC) and a solid-state catheter-tip transducer during electrical nerve stimulation in the gracilis muscle of anesthetized dogs. MBF values obtained simultaneously by the HTC method and the inhalated hydrogen gas (H2) clearance method showed an excellent linear regression with a correlation coefficient of 0.93. Electrical nerve stimulation at frequencies of 5 and 100 Hz increased total MBF, determined by an electromagnetic flowmeter, over control values 6.57 +/- 0.82 and 6.32 +/- 0.93 ml/min/100 g, respectively. Regional MBF at 5 Hz stimulation determined with HTC increased in the same magnitude both at the midpoint and the distal portion of the gracilis muscle. However, following 100 Hz stimulation, MBF at the midpoint was decreased from 8.15 +/- 1.55 to 4.09 +/- 1.54 ml/min/100 g, whereas at the distal portion flow was increased from 6.39 +/- 1.71 to 21.2 +/- 5.29 ml/min/100 g. IMP in both regions was low and approximately 2.0 mmHg at rest. IMP was remarkably increased to 27.9 +/- 8.43 mmHg at the midpoint but not in the distal portion during 100 Hz tetanic contraction, but showed no significant change in either region following 5 Hz stimulation. These data suggest that HTC can serve to measure MBF, and that following 100 Hz tetanic contraction, increased IMP may be associated with decreased regional MBF, irrespective of a total increase in flow in the gracilis muscle.
在麻醉犬的股薄肌进行电神经刺激期间，使用热热电偶（HTC）和固态导管尖端换能器测量骨骼肌血流量（MBF）和肌内压（IMP）。通过HTC方法和吸入氢气（H2）清除法同时获得的MBF值显示出良好的线性回归，相关系数为0.93。频率为5和100 Hz的电神经刺激使通过电磁流量计测定的总MBF分别比对照值6.57±0.82和6.32±0.93 ml/min/100 g增加。用HTC测定的5 Hz刺激时的局部MBF在股薄肌中点和远端部分均以相同幅度增加。然而，在100 Hz刺激后，中点处的MBF从8.15±1.55降至4.09±1.54 ml/min/100 g，而远端部分的血流量从6.39±1.71增加至21.2±5.29 ml/min/100 g。两个区域的IMP在静息时均较低，约为2.0 mmHg。在100 Hz强直收缩期间，中点处的IMP显著增加至27.9±8.43 mmHg，而远端部分未增加，但在5 Hz刺激后两个区域均无明显变化。这些数据表明HTC可用于测量MBF，并且在100 Hz强直收缩后，IMP升高可能与局部MBF降低有关，而与股薄肌血流量的总体增加无关。
