Fujimura Koji, Okada Norihiro
Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Dev Growth Differ. 2007 May;49(4):301-24. doi: 10.1111/j.1440-169X.2007.00926.x.
We described the developmental stages for the embryonic, larval and early juvenile periods of Nile tilapia Oreochromis niloticus to elucidate sequential events of craniofacial development. Craniofacial development of cichlids, especially differentiation and morphogenesis of the pharyngeal skeleton, progresses until about 30 days postfertilization (dpf). Because there is no comprehensive report describing the sequential processes of craniofacial development up to 30 dpf, we newly defined 32 stages using a numbered staging system. For embryonic development, we defined 18 stages (stages 1-18), which were grouped into seven periods named the zygote, cleavage, blastula, gastrula, segmentation, pharyngula and hatching periods. For larval development, we defined seven stages (stages 19-25), which were grouped into two periods, early larval and late larval. For juvenile development until 30 dpf, we defined seven stages (stages 26-32) in the early juvenile period. This developmental staging system for Nile tilapia O. niloticus will benefit researchers investigating skeletogenesis throughout tilapia ontogeny and will also facilitate comparative evolutionary developmental biology studies of haplochromine cichlids, which comprise the species flocks of Lakes Malawi and Victoria.
我们描述了尼罗罗非鱼（Oreochromis niloticus）胚胎期、幼体期和幼鱼早期的发育阶段，以阐明颅面发育的连续事件。丽鱼科鱼类的颅面发育，尤其是咽骨骼的分化和形态发生，在受精后约30天（dpf）之前持续进行。由于尚无全面描述直至30 dpf颅面发育连续过程的报告，我们使用编号分期系统新定义了32个阶段。对于胚胎发育，我们定义了18个阶段（1-18阶段），这些阶段被分为七个时期，即合子期、卵裂期、囊胚期、原肠胚期、体节期、咽胚期和孵化期。对于幼体发育，我们定义了七个阶段（19-25阶段），这些阶段被分为两个时期，即幼体早期和幼体晚期。对于直至30 dpf的幼鱼发育，我们在幼鱼早期定义了七个阶段（26-32阶段）。尼罗罗非鱼的这种发育分期系统将有助于研究罗非鱼个体发育过程中骨骼发生的研究人员，也将促进对构成马拉维和维多利亚湖物种群的帚齿丽鱼科丽鱼的比较进化发育生物学研究。
