Zhigang Zhao, Wenlu Shen
Department of Urology, The Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China.
Prostate. 2007 Aug 1;67(11):1143-51. doi: 10.1002/pros.20610.
Prior data clearly demonstrated the expression of prostate stem cell antigen (PSCA) mRNA in prostatic intraepithelial neoplasia (PIN) tissues. The purpose of the present investigation was to determine whether PSCA mRNA expression was associated with the presence of cancer in this disease.
One hundred seventeen men were diagnosed with isolated PIN on initial prostate biopsy, 51 with low-grade form (LGPIN), and 66 with high-grade form (HGPIN). PSCA mRNA expression in initial PIN and subsequent cancer was examined by in situ hybridization (ISH). The differences of the PSCA mRNA expression level between the groups were analyzed by the Chi-square and Student's t-test. Univariate and multivariate logistic regression analyses were performed to evaluate the predictive performance of PSCA mRNA.
PSCA mRNA expression level in 34 subsequent cancers was statistically increased compared with their paired PIN (P < 0.001), with a Gleason's dependence. HGPIN showed statistically high PSCA mRNA expression compared with LGPIN (P < 0.01). PSCA mRNA expression levels were significantly stronger in the initial isolated LGPIN and isolated HGPIN with subsequent cancer than those without (P < 0.001 and P < 0.001, respectively). Multivariate logistic regression analysis demonstrated that only PSCA mRNA was predictive of the onset of subsequent cancer in patients with isolated LGPIN and in those with isolated HGPIN, respectively.
Our data identify PSCA mRNA in initial PIN as a significant predictor of subsequent cancer, suggesting that PSCA implies in prostatic tumorigenesis and may be used to identify the patients with isolated PIN who are at high risk for cancer onset in the disease process.
既往数据清楚地表明前列腺干细胞抗原（PSCA）mRNA在前列腺上皮内瘤变（PIN）组织中表达。本研究的目的是确定PSCA mRNA表达是否与该疾病中的癌症存在相关。
117名男性在初次前列腺活检时被诊断为孤立性PIN，其中51例为低级别形式（LGPIN），66例为高级别形式（HGPIN）。通过原位杂交（ISH）检测初次PIN及后续癌症中PSCA mRNA的表达。采用卡方检验和Student's t检验分析各组间PSCA mRNA表达水平的差异。进行单因素和多因素逻辑回归分析以评估PSCA mRNA的预测性能。
34例后续癌症中PSCA mRNA表达水平与其配对的PIN相比有统计学意义的升高（P < 0.001），与Gleason分级相关。与LGPIN相比，HGPIN显示出统计学意义的高PSCA mRNA表达（P < 0.01）。初始孤立的LGPIN和孤立的HGPIN且后续发生癌症者的PSCA mRNA表达水平明显强于未发生癌症者（分别为P < 0.001和P < 0.001）。多因素逻辑回归分析表明，仅PSCA mRNA分别可预测孤立性LGPIN患者和孤立性HGPIN患者后续癌症的发生。
我们的数据表明初次PIN中的PSCA mRNA是后续癌症的重要预测指标，提示PSCA参与前列腺肿瘤发生，可用于识别在疾病过程中发生癌症风险高的孤立性PIN患者。
