嗜热栖热菌DNA聚合酶的结晶及初步晶体学研究
Crystallization and preliminary crystallographic study of DNA polymerase from Pyrococcus furiosus.
作者信息
Nishida Hirokazu, Tanabe Maiko, Ishino Yoshizumi, Oyama Takuji, Morikawa Kosuke
机构信息
Central Research Lab, Hitachi Ltd, Kokubunji, Tokyo, Japan.
出版信息
Protein Pept Lett. 2007;14(4):403-5. doi: 10.2174/092986607780363853.
A new member of archaeal DNA polymerase from Pyrococcus furiosus was crystallized. Diffraction data to 3.1 A of the selenomethionine-derivatized crystal were collected, and preliminary crystallographic study has been completed. The crystal belongs to the space group C2 with unit cell parameters of a = 93.2 A, b = 124.9 A, c = 87.7 A, alpha = 90 degrees , beta = 109.7 degrees , and gamma = 90 degrees . Assuming the presence of one molecule in the asymmetric unit, the solvent content of the crystal is estimated to be 54%, corresponding to a Matthews coefficient V(M) of 2.7A (3) Da(-1).
来自嗜热栖热菌的古菌DNA聚合酶的一个新成员被结晶。收集了硒代甲硫氨酸衍生化晶体至3.1埃的衍射数据，并完成了初步晶体学研究。该晶体属于空间群C2，晶胞参数为a = 93.2埃，b = 124.9埃，c = 87.7埃，α = 90°，β = 109.7°，γ = 90°。假设不对称单元中存在一个分子，该晶体的溶剂含量估计为54%，对应于马修斯系数V(M)为2.7埃³道尔顿⁻¹。
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