Kang I S, Koong M K, Forman J, Siler-Khodr T M
Department of Obstetrics and Gynecology, University of Texas Health Science Center, San Antonio 78284.
Am J Obstet Gynecol. 1991 Dec;165(6 Pt 1):1771-6. doi: 10.1016/0002-9378(91)90031-l.
The dose-related effect of gonadotropin-releasing hormone on placental prostanoids was studied with a perifusion system. Villous tissues were perifused with medium 199 (1 ml/hr) and at the beginning of the fifth hour, either 0, 10(-10), 10(-9), 10(-8), 10(-7), or 10(-6) mol/L gonadotropin-releasing hormone was added to the medium of triplicate chambers. The concentration of prostaglandin E, prostaglandin F, 13,14-dihydro-15-keto-prostaglandin F2 alpha, 6-keto-prostaglandin F1 alpha, and thromboxane B2 in the effluent medium, collected every hour, was determined by specific radioimmunoassay. The cumulative release after gonadotropin-releasing hormone treatment for each chamber was calculated, and replicate chambers were averaged. Linear regression analysis of the average for each dose from three different placentas was used to determine the dose-response relationship. Gonadotropin-releasing hormone significantly inhibited the release of placental prostaglandin E, prostaglandin F, and thromboxane B2 in a dose-dependent fashion. Gonadotropin-releasing hormone had no significant effect on 13,14-dihydro-15-keto-prostaglandin F2 alpha and 6-keto-prostaglandin F1 alpha, although there was an apparent increase in 13,14-dihydro-15-keto-prostaglandin F2 alpha. These data support the hypothesis that chorionic gonadotropin-releasing hormone inhibits prostanoid production from the placenta, which in turn may regulate various functions of prostanoids during pregnancy.
采用灌流系统研究了促性腺激素释放激素对胎盘前列腺素的剂量相关效应。用199培养基（1毫升/小时）对绒毛组织进行灌流，在第五小时开始时，向一式三份培养室的培养基中分别加入0、10(-10)、10(-9)、10(-8)、10(-7)或10(-6)摩尔/升的促性腺激素释放激素。每小时收集流出培养基，采用特异性放射免疫分析法测定前列腺素E、前列腺素F、13,14-二氢-15-酮-前列腺素F2α、6-酮-前列腺素F1α和血栓素B2的浓度。计算每个培养室在促性腺激素释放激素处理后的累积释放量，并对重复培养室的数据求平均值。对来自三个不同胎盘的每个剂量的平均值进行线性回归分析，以确定剂量反应关系。促性腺激素释放激素以剂量依赖方式显著抑制胎盘前列腺素E、前列腺素F和血栓素B2的释放。促性腺激素释放激素对13,14-二氢-15-酮-前列腺素F2α和6-酮-前列腺素F1α无显著影响，尽管13,14-二氢-15-酮-前列腺素F2α有明显增加。这些数据支持以下假设：绒毛膜促性腺激素释放激素抑制胎盘前列腺素的产生，这反过来可能在怀孕期间调节前列腺素的各种功能。
