Gal D, Strickland D M, Lifshitz S, Buchsbaum H J, Mitchell M D
Int J Radiat Oncol Biol Phys. 1984 May;10(5):653-7. doi: 10.1016/0360-3016(84)90296-7.
The effect of gamma irradiation on the production of prostaglandins by human colon was investigated. Squares of tissue in organ culture dishes were irradiated with 500, 1000, or 2500 rad in single applications. Tissues that were not irradiated served as controls. After treatment the tissues were superfused and prostaglandin concentrations in the effluent fluid were determined. The rates of production of prostaglandins E2 and F2 alpha by irradiated tissues were significantly lower (p less than 0.05) than those of nonirradiated tissues. Neither the release of lactate dehydrogenase nor the rate of production of 13,14-dihydro-15-keto-prostaglandin F2 alpha were increased in the irradiated samples, suggesting that neither decreased cell viability nor increased prostaglandin metabolism accounted for the decreased prostaglandin production rates. We conclude that irradiation of the human colon in vitro results in an acute inhibition of prostaglandin synthesis. The cytoprotective nature of prostaglandins is discussed with regard to the possible pathophysiological significance of these findings.
研究了γ射线照射对人结肠前列腺素生成的影响。将器官培养皿中的组织方块单次给予500、1000或2500拉德的照射剂量。未照射的组织作为对照。处理后，对组织进行超灌流，并测定流出液中前列腺素的浓度。照射组织中前列腺素E2和F2α的生成速率显著低于未照射组织（p＜0.05）。照射样本中乳酸脱氢酶的释放及13,14-二氢-15-酮-前列腺素F2α的生成速率均未增加，这表明前列腺素生成速率降低既不是由于细胞活力下降，也不是由于前列腺素代谢增加所致。我们得出结论，体外照射人结肠会导致前列腺素合成的急性抑制。就这些发现可能的病理生理意义，讨论了前列腺素的细胞保护性质。
