Perciani C T, Peixoto P S, Dias W O, Kubrusly F S, Tanizaki M M
Centro de Biotecnologia, Instituto Butantan, São Paulo, Brazil.
J Clin Lab Anal. 2007;21(3):201-6. doi: 10.1002/jcla.20172.
The methods currently used to determine the immunoglobulin avidity index (AI) require the choice of a reference point in the ELISA titration curve. Since both curves, with and without denaturating reagents, seldom run in parallel, the AI value becomes highly dependent on this reference. The new method for AI calculation presented here takes into account the whole data of the ELISA titration curve in which the final numerical AI is the average of each point.
目前用于确定免疫球蛋白亲和力指数(AI)的方法需要在酶联免疫吸附测定(ELISA)滴定曲线中选择一个参考点。由于使用和不使用变性试剂时的两条曲线很少平行,AI值变得高度依赖于此参考点。本文提出的AI计算新方法考虑了ELISA滴定曲线的全部数据,其中最终的数值AI是每个点的平均值。